Nitrosomonas europaeaのアンモニア酸化系に及ぼす各種添加物の影響

概要

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The effects of additives on the ammonia-oxidative system of Nitrosomonas europaea were studied. The addition of 1 mM MgCl_2 or 5×10^<-5> mM ATP to fragments of the cell bodies activated the oxidation of ammonia and hydroxylamine; the effect of Mg^<2+>+ATP on the oxidation of ammonia was particularly striking. DAAE-cellulose fractions obtained from the soluble portion of the cell bodies (F.1 to F.5)added to fragments of the cell bodies also activated the oxidation of ammonia hydroxylamine. Of these fractions F.3 was most potent.The addition of 1 mM MgCl_2 or 5×10<-5>mM ATP resulted in activation of hydroxylamine oxidation by F.1 and F.2 obtained from DEAE-cellulose column. The addition of these substances to the fractions resulted in no activation of ammonia oxidation.
社団法人日本生物工学会の論文