Nitrosomonas europaeaの無細胞抽出液によるアンモニアおよびヒドロキシルアミンの酸化

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Five protein fractions with hydroxylamine-oxidizing activity(F.1 to F.5)were partially purified from the cell-free extract of Nitrosomonas europaea, and their effects of the oxidation of hydroxylamine and ammonia were studied. Fraction F.3,which showed the highest activity for oxidation of hydroxylamine, was purified about 102-fold compared with the crude extract by chromatography on Sephadex G-100. Hydroxylamine oxidase activity in combinations of the five protein fractions were measured, and the combination of F.3+F.5 proved to have the highest activity. The suspension (A) of CF.2 with ammonia-oxidizing activity was combined with each of the five protein fractions, and ammonia-oxidizing and hydroxylamine-oxidizing activities of the suspension were measured. The suspension had higher ammonia oxidizing alone than in combination with any of the protein fractions, but had high hydroxy-lamine-oxidizing activity in combination with F.5.
社団法人日本生物工学会の論文