タバコ立枯病菌の放射性りん(^<32>P)による標識法について

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Method for labe1ing bacteria with radioactive phosphorus (^32P) was Studied using Pseudomonas solanacearum E. F. Smith, the causal agent of tobacco wilt disease. Potato sucrose 1iquid medium (PS) and bouillon medium(B), both added with lμc/m1 of ^32p, were compared each other as to the bacterial growth and the uptake of 32P by the bacteria. The PS culture medium was found to be more satisfactory for the bacterial growth and the uptake of ^32P. The amount of uptake of ^32P by bacteria was markedly reduced by the addition of non-radioactive phosphorus(^31P) to the PS medium. The amount of ^32P uptake per bacterial ce11 was less in the higher bacterial concentration of inoculum (10^8/ml) than that of the 1ower one(10^6/ml) in the PS medium for 5hours at 30℃. In this experiment, a high degree of efficiency of ^32P uptake by total bacteria was obtained in culture containing 10^7 ce11s per ml for 48 hours at 30℃. The increase of the ^32P uptake per bacterial ce11 was fo11owed by the increased quantity of ^32P in the medium containing from 10^-1μc per ml to 10μc per ml, for 48 hours at 30℃. Preservation of ^32 P labeled bacteria in three non-radioactive media (water, culture so1ution for tobacco and PS medium) was examined at 10℃ and 30℃. Genera11y, the higher rate of release of ^32P from the labeled bacteria was shown in the incubation at 30℃ and the 1owest released rate was shown in the culture solution for to bacco. The rate of released ^32P from labeled bacteria in the culture solution at 30℃ was found as follows: after one day-about l%, 3days-about 2%, 5 and 10 days-about3%.(Received June 11,1965)
日本植物病理学会の論文
1966-01-30

タバコ立枯病菌の放射性りん(^<32>P)による標識法について

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