キュウリ子葉におけるタバコモザイクウイルスの増殖と局在化に及ぼす各種薬剤の影響

概要

論文の詳細を見る
The effect of metabolic inhibitors on multiplication and distribution of tobacco mosaic virus (TMV) and cucumber green mottle mosaic virus (CGMMV) in cucumber cotyledons was investigated. The amount of TMV, as assayed by ELISA, increaed about 8 times in cucumber cotyledon when actinomycin D (3 μg/ml) was applied immediately or at 1 day after inoculation, but not increased by the application at 2 or 3 days after inoculation. TMV also increased to 3-fold when rifampicin (100 μg/ml) was applied just after inoculation. These antibiotics had no significant effect on CGMMV multiplication in cucumber cotyledons. The area of TMV distribution in cucumber coty ledons was researched using the fluorescent antibody technique. TMV was observed in limited areas of the epidermal and palisade layers when cotyledons were treated with water. When cotyledons were treated with actinomycin D, however, TMV multiplied and spread horizontally and vertically in the tissues, and infected cells were observed in the upper epidermal, palisade, spongy parenchyma and also lower epidermal layers. These results revealed that actinomycin D increased TMV concetration in consequence of the spread of infected areas by inhibiting localizaion to the virus.
日本植物病理学会の論文
1989-04-25