キャベツ葉組織における病原性・非病原性細菌の増殖と組織の抗菌活性

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To elucidate the mechanisms of resistance to black rot disease of cabbage, the relationship between multiplication of bacteria and the activity of antibacterial substances in cabbage leaves inoculated with a compatible bacterium (Xanthomonas campestris pv. campestris, Xcc), an incompatible bacterium (X. campestris pv. phaseoli, Xcp) which is pathogenic to kidney bean plant, and a saprophyte (Serratia marcescens, Sm) was investigated. When the multiplication rate of these bacteria was examined using the two inoculation methods of needle-prick and injection, Xcc multiplied rapidly showing a logarithmic curve and formed typical lesions, but multiplication of Xcp and Sm were severely inhibited from a nearly stage of inoculation and their viable cells gradually decreased in number. The intercellular fluid obtained from healthy cabbage leaves, however, supported good multiplication of all three bacteria, and an inhibitory effect not specific to uncongenial bacteria was observed. Water extract and ethyl acetate extract obtained from the leaves 3 days after inoculation with Xcp showed obviously higher antibacterial activity than that obtained from healthy leaves. These active substances in the water extract were dialyzed through a cellophane membrane, and therefore it was suggested that the substances may be low in molecular weight. The ethyl acetate-extractable antibacterial substances were detected at a high level in the leaves as early as one day after inoculation with Xcp, and its concentration was maintained for 7 days. Almost the same production pattern of antibacterial substances was observed in leaves inoculated with Sm, but the amount was considerably lower. In the leaves inoculated with Xcc, on the contrary, these substances did not increase until the time when lesions became visible, increased gradually as lesions enlarged, and reached a considerably high level at the later stage of symptom development. Small quantities of these antibacterial substances were also found in healthy leaves.
日本植物病理学会の論文
1986-10-25

キャベツ葉組織における病原性・非病原性細菌の増殖と組織の抗菌活性

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