オギで見出された双球状ウイルス;オギ条斑ウイルス (miscanthus streak virus, MiSV) について

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A previously undescribed geminivirus, miscanthus streak virus (MiSV), was found in dip preparations from Miscanthus sacchariflorus Benth. et Hook. showing leaf streak and stunting, collected at Matsudo of Chiba prefecture in 1978. The virus was purified by homogenization using 0.1 M phosphate buffer (pH 5.6) containing 0.1% thioglycollic acid and 1% triton X-100, followed by chloroform clarification (20%), and then three cycles of differential centrifugations. The virus was purified as a well-defined band by sucrose density gradient centrifugation. The purified preparations showed an UV absorption spectrum characteristic of a nucleoprotein. The sedimentation coefficient of the virus determined by analytical centrifugation was about 75 S. The virus particles were about 18×30 nm in size and contained DNA suggested by diphenyl amine test. The DNA was suggested to be single-strand. In agar gel diffusion test, the antiserum (titer 128) of the virus formed a single band against the homologous antigen. The virus was serologically unrelated to maize streak virus and chloris striate mosaic virus of leafhopper-borne geminiviruses, and to cassava latent virus and tobacco leaf curl virus of whitefly-borne geminiviruses. The virus particles were observed as singles or aggregates in the nucleus, cytoplasm and vacuole of various cells. Obvious paired particles were often found in the nucleus and cytoplasm. In the vacuole, geometrical crystalline arrays formed from paired particles were also observed. So, the paired particles were considered to be natural nature of the virus. The virus was not transmitted by sap inoculation, and its vector was not known so far.
日本植物病理学会の論文
1985-12-25

オギで見出された双球状ウイルス;オギ条斑ウイルス (miscanthus streak virus, MiSV) について

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