ロケット免疫電気泳動 (RIE) 法による植物ウイルスの検出, 定量

概要

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Rocket immunoelectrophoresis (RIE) assay was applided for 9 plant viruses, barley stripe mosaic virus, tobacco mosaic virus, white clover mosaic virus, bean yellow mosaic virus, rice stripe virus, rice dwarf virus, cucumber mosaic virus, tobacco necrosis virus and its satellite virus. Agarose gels containing 4 mM barbital, 20 mM barbital sodium, 2 mM EDTA, 200μg/ml sodium lauryl sulfate, 0.05-0,1% crude antiserum and 1% agarose, pH 8.6 were useful for virus assay. Barley stripe mosaic virus, tobbacco mosaic virus and rice stripe virus were detected in 0.5μg/ml, 1μg/ml and 0.05 (in A_<250> value) of purified preparations, and in 1/256, 1/320 and 1/128 (W/V) crude saps from infected plants, respectively. Barley stripe mosaic virus was detected in about 250 protoplasts isolated from in fected barley plants. The assay required only 5 to 7 hours for electrophoresis and staining. When two viruses were electrophoresed in the gel containing both antiserum, rocket-shaped precipitates from the same well were formed and each virus was assayed simultaneously.
日本植物病理学会の論文
1984-10-25