Isolation of GnafC, a Polysaccharide Constituent of Gnaphalium affine, and Synergistic Effects of GnafC and Ascorbate on the Phenotypic Expression of Osteoblastic MC3T3-E1 Cells(Food & Nutrition Science)
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概要
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After screening extensively factors in plant extracts that increase alkaline phosphatase activity, an osteoblastic differentiation marker protein in mouse calvarial osteoblast MC3T3-E1 cells, GnafC derived from Gnaphalium affine, was found to significantly enhance the alkaline phosphatase (ALPase) activity in a synergistic manner with ascorbate. GnafC was a polysaccharaide with an approximate molecular mass of 10,000 and comprised mannose, xylose, arabinose, galactose and glucose in a molar ratio of 1:2:4.3:2.5:2.7. Expression of the osteoblastic differentiation marker genes was examined by semiquantitative RT-PCR with RNAs prepared from cells at different developmental stages. With ascorbate in the culture, GnafC enhanced the expression of the ALPase and MMP13 genes from the early stage of differentiation, leading to maturation of the collagenous extracellular matrix (ECM), a prerequisite for mineralization.
- 社団法人日本農芸化学会の論文
- 2003-10-23
著者
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Hasegawa Yoshinori
Department of Respiratory Medicine, Nagoya University Graduate School of Medicine, Japan
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鳥居 恭好
名古屋大学生命農学研究科
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NAGASAKA Atsushi
Department of Gastroenterology, Sapporo Municipal Hospital
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Torii Yasuko
Basic High-technology Laboratories Sumitomo Electric Industries Ltd.
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鳥居 恭好
日大生資科
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Torii Yasuyoshi
Department Of Food Science And Technology Collage Of Bioresource Sciences Nihon University
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AOSHIMA Yukihiro
Department of Biological Mechanisms and Functions, Graduate School of Bioagricultural Sciences, Nago
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HASEGAWA Sho
Department of Biological Mechanisms and Functions, Graduate School of Bioagricultural Sciences, Nago
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KIMURA Tomofumi
Department of Biological Mechanisms and Functions, Graduate School of Bioagricultural Sciences, Nago
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HASHIMOTO Satofumi
Department of Biological Mechanisms and Functions, Graduate School of Bioagricultural Sciences, Nago
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TSUKAGOSHI Norihiro
Aichi Study Center, The University of the Air
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Hasegawa Yoshinori
Department Of Biological Mechanisms And Functions Graduate School Of Bioagricultural Sciences Nagoya
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Hasegawa Sho
Department Of Biological Mechanisms And Functions Graduate School Of Bioagricultural Sciences Nagoya
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Kimura Tomofumi
Department Of Biological Mechanisms And Functions Graduate School Of Bioagricultural Sciences Nagoya
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Nagasaka Atsushi
Department Of Biological Mechanisms And Functions Graduate School Of Bioagricultural Sciences Nagoya
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Aoshima Yukihiro
Department Of Biological Mechanisms And Functions Graduate School Of Bioagricultural Sciences Nagoya
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Hashimoto Satofumi
Department Of Biological Mechanisms And Functions Graduate School Of Bioagricultural Sciences Nagoya
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Tsukagoshi Norihiro
Aichi Study Center The University Of The Air
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