The Effect of in Vitro and in Vivo Ethylenbis Dithiocarbamate Fungicides on NMDA Receptors in Rat Brain Membranes
スポンサーリンク
概要
- 論文の詳細を見る
To determine whether the ethlenbisdithiocarbamate fungicides, zineb, manzeb and maneb affect the N-methyl-D-aspartate (NMDA) receptor in rat brain membranes, we performed a binding assay using [^3H]MK-801, a noncompetitive NMDA receptor antagonist. Displacement studies were conducted using well washed membranes to exclude the effect of endogenous acidic amino acids on the binding of [^3H]MK-801. In both the presence or absence of added glutamate and glycine in the assay buffer, the dose-response curve indicated that zineb enhanced the binding in a concentration range of 100-500μM. However, the displacement curves indicated that manzeb and maneb inhibited the binding in a concentration range of 10-500μM. The addition of 50μM glutamate and glycine to the assay medium increased binding by 5-20% above the control in a concentration range of 0.1-100μM. No rats injected with zineb, manzeb, maneb (100 mg/kg, ip) showed any characteristic toxic signs or any significant weight changes within 24 hrs. Estimation of [^3H]MK-801 binding to unwashed membranes from intoxicated rat brains revealed no marked change in Bmax or Kd values for 24 hrs following fungicide administration.
- 日本衛生学会の論文
著者
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TSUNODA Masashi
Department of Preventive Medicine and Public Health, Kitasato University School of Medicine
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Konno N
Koriyama Women's Univ. And Coll. Koriyama Jpn
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Konno Nobuhiro
Department Of Public Health Fukushima Medical University School Of Medicine
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Tsunoda Masashi
Department Of Preventive Medicine And Public Health Kitasato University School Of Medicine
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Tsunoda Masashi
Department Of Hygiene Kitasato University School Of Medicine
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Tsunoda M
Fukushima Medical Univ. Fukushima Jpn
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NAKANO Kyoko
Department of Public Health, Fukushima Medical University, School of Medicine
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Nakano Kyoko
Department Of Medical Technology Hirosaki University School Of Health Sciences
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Nakano Kyoko
Department Of Public Health Fukushima Medical University School Of Medicine
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Konno Nobuhiro
Department Of Public Health Fukushima Medical College
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