Development of an Effective Sample Preparation Method for the Proteome Analysis of Body Fluids Using 2-D Gel Electrophoresis(Biochemistry & Molecular Biology)
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概要
- 論文の詳細を見る
A sample preparation is still the most critical step in two-dimensional electrophoresis (2-DE) and should be optimized for each type of sample. In this study, a protein extraction method from body fluids was developed using a combined centrifugal filter device and a sample treating buffer. When plasma, amniotic fluid, urine, and tear were tested with this method, the recovery of protein reached almost 90% and high-quality separation of 2-DE gel was obtained.
- 社団法人日本農芸化学会の論文
- 2003-07-23
著者
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KIM Chan-Wha
School of Life Sciences and Biotechnology, Korea University
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Kim C‐w
Korea Univ. Seoul Kor
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Kim Chan-wha
Graduate School Of Life Sciences And Biotechnology Korea University
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Kim Chan-wha
Graduate School Of Biotechnology Korea University
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LEE Do-Youn
Graduate School of Life Sciences and Biotechnology, Korea University
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JOO Won-A
Graduate School of Life Sciences and Biotechnology, Korea University
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Kim Chan-shick
Graduate School Of Life Sciences And Biotechnology Korea University
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Lee Do-youn
Graduate School Of Life Sciences And Biotechnology Korea University
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Joo Won-a
Graduate School Of Life Sciences And Biotechnology Korea University
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Joo W‐a
Graduate School Of Life Sciences And Biotechnology Korea University
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