High-level TNF-α Secretion and Macrophage Activity with Soluble β-Glucans from Saccharomyces cerevisiae(Food & Nutrition Science)
スポンサーリンク
概要
- 論文の詳細を見る
We have previously reported that water-soluble β-glucan completely devoid of mannoprotein and purified from the yeast cell wall effectively stimulated the macrophage function (Biosci. Biotechnol, Biochem., 65, 4, 837-841 (2001)). In this present study, to increase the yield of water-soluble β-glucan, the wild type of Sacharomyces cerevisiae, JH, was treated with a combination of UV irradiation and laminarinase (endo-β-(1,3)-glucanase) to yield the laminarinase-resistant mutants, JUL1 and JUL3. Water-soluble β-glucans that were free of mannoprotein from JH, JUL1 and JUL3 were purified and their effects on TNF-α secretion and phagocytosis by macrophages were evaluated. Crude β-glucan was first solubilized from the yeast cell wall by alkaline extraction and then subjected to an acid treatment. The residual mannoprotein was completely removed by DEAE and ConA chromatography. The yield of water-soluble β-glucan in both mutants (JUL1, 5.11% ; JUL3, 5.76%) was about 5-fold higher than that of the wild type (1.16%). The water-soluble β-glucan from JH induced TNF-a secretion slightly more than that from JUL1 or JUL3 : TNF-α secretion by JH at 50, 200, 500 μg/ml ofβ-glucan was 11-17% more than that by JUL1 or JUL3 for the same treatment. β-Glucan from the wild type stimulated phagocytosis slightly more than that from the mutants. These mutants could therefore effectively produce purified water-soluble β-glucan with immune activity.
- 社団法人日本農芸化学会の論文
- 2002-02-23
著者
-
KIM Chan-Wha
School of Life Sciences and Biotechnology, Korea University
-
Kim C‐w
Korea Univ. Seoul Kor
-
Kim Chan-wha
Graduate School Of Life Sciences And Biotechnology Korea University
-
Kim Chan-wha
Graduate School Of Biotechnology Korea University
-
LEE Do-Youn
Graduate School of Life Sciences and Biotechnology, Korea University
-
Chang Hyo-ihl
Graduate School Of Biotechnology Korea University
-
Kim Chan-shick
Graduate School Of Life Sciences And Biotechnology Korea University
-
Lee Do-youn
Graduate School Of Life Sciences And Biotechnology Korea University
-
JI In-Hye
Graduate School of Biotechnology, Korea University
-
Ji In-hye
Graduate School Of Biotechnology Korea University
関連論文
- Enhanced Hypoglycemic Activity Following Intratracheal Administration of Insulin Microcrystal Suspension with Injection Adjuvant
- Stabilization of Glycoprotein Liquid Formulation Using Arginine: A Study with Lactoferrin as a Model Protein
- Analysis of Differentially Regulated Proteins in TM4 Cells Treated with Bisphenol A
- Characterization of Streptozotocin-induced Diabetic Rats and Pharmacodynamics of Insulin Formulations(Biochemistry & Molecular Biology)
- In Vitro Model using Mouse Hepatocytes for Study of Alcohol Stress(Biochemistry & Molecular Biology)
- Amino Acid Sequence Analysis of Bitter Peptides from a Soybean Proglycinin Subunit Synthesized in Escherichia coli
- An Approach to Strain Improvement and Enhanced Production of Clavulanic Acid in Streptomyces clavuligerus
- High-level TNF-α Secretion and Macrophage Activity with Soluble β-Glucans from Saccharomyces cerevisiae(Food & Nutrition Science)
- Development of an Effective Sample Preparation Method for the Proteome Analysis of Body Fluids Using 2-D Gel Electrophoresis(Biochemistry & Molecular Biology)
- Purification of Soluble β-Glucan with Immune-enhancing Activity from the Cell Wall of Yeast
- Proteomics of Halophilic archaea
- Crystallization of Antiangiogenic Kringle V Derived from Human Apolipoprotein A : Crystallization Applied to Purification and Formulation
- Establishment of a Cryopreservation Method for the Industrial Use of D-Amino Acid Oxidase-Overexpressing Escherichia coli
- An Approach to Strain Improvement and Enhanced Production of Clavulanic Acid in Streptomyces clavuligerus
- Stabilization of Glycoprotein Liquid Formulation Using Arginine : A Study with Lactoferrin as a Model Protein
- Differentially Expressed Proteins of Mesenchymal Stem Cells Derived from Human Cord Blood (hUCB) during Osteogenic Differentiation
- Sample preparation method for plasma membrane proteome analysis
- Human blood plasma preparation for two-dimensional gel electrophoresis
- Microencapsulation of Insulin Microcrystals