Ribonuclease Inhibitors in Malus x domestica (Common Apple) : Isolation and Partial Characterization(Biochemistry & Molecular Biology)
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概要
- 論文の詳細を見る
A ribonuclease inhibitory activity was detected in the fruits of common apple, Malus x domestica, cv. Fuji, and purified by affinity chromatography on ribonuclease A-Sepharose. It inhibited hydrolysis of cyclic-2' : 3'-CMP by bovine pancreatic ribonuclease A with an apparent inhibition constant of about 5 × 10^<-8>M. Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry of the purified protein gave two peaks corresponding to the mass numbers of 55,658 and 62,839, while three bands of 43-, 34-, and 21-kDa were detected by SDS-PAGE. These results suggested that the inhibitor preparation was a mixture of two proteins comprised of 43- and 21-kDa subunits or of 34- and 21-kDa subunits. Attempts to separate these two proteins were unsuccessful. Amino acid composition and N-terminal amino acid sequence of these subunits were also identified and N-terminal sequences showed some similarity to that of cottonseed storage globulin. The significance of the presence of ribonuclease inhibitors in apple fruits is not clear, but it might allow some speculation about their possible involvement in the control of the self-incompatibility ribonuclease of Rosaceae plants.
- 社団法人日本農芸化学会の論文
- 2003-04-23
著者
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Odani Shoji
Department Of Biology Faculty Of Science Niigata University
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TAKAHASHI Yoshiaki
Department of Applied Chemistry, Nagoya University
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Isemura Mamoru
Department Of Cellular Biochemistry School Of Food And Nutritional Sciences University Of Shizuoka
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Kosuge Takao
Department Of Biology Faculty Of Science Niigata University
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Kosuge Takao
Department Of Applied Chemistry Faculty Of Engineering Tokai University
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ODANI Sumiko
Department of Home Economics, Faculty of Education and Human Science, Niigata University
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Takahashi Y
Department Of Medical Technology School Of Health Science Faculty Of Medicine Niigata University
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Odani Shoji
Department Biology Faculty Of Science Niigata University
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Odani Sumiko
Department Of Home Economics Faculty Of Education And Human Science Niigata University
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Takahashi Yoshiaki
Department Of Anatomy Oita Medical University
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