Molecular Cloning and Sequence Analysis of a Gene Encoding an Extracellular Serine Protease from Streptomyces lividans 66
スポンサーリンク
概要
- 論文の詳細を見る
A gene encoding a homolog of the chymotrypsin-like serine protease (SAM-P20), which was isolated as the target enzyme of a protease inhibitor (SSI), was cloned from Streptomyces lividans 66. This gene contained an open reading frame of 1065 nucleotides encoding 354 amino acid residues with a putative prepro portion of 157 amino acid residues. The deduced amino acid sequence of the cloned gene had significant homology to those of members of Streptomyces extracellular chymotrypsin-like protease family. By Southern blot analysis, it was suggested that protease genes of this type are found at a high frequency in Streptomyces. In this sense, we propose to categorize this protease as a member of the `SAL' series (SAM-P20-like proteases).
- 社団法人日本農芸化学会の論文
- 1995-07-23
著者
-
ENDO Takeshi
Department of Chemistry, Sophia University
-
Endo T
Hoshi Univ. Tokyo Jpn
-
MOMOSE HARUO
Department of Biological Science and Technology, Science University of Tokyo
-
TAGUCHI Seiichi
Department of Biological Science and Technology, Science University of Tokyo
-
Endo T
Chiba Univ.
-
Endo Takeshi
Department Of Biology Faculty Of Science Chiba University
-
Momose H
Sci. Univ. Tokyo Chiba
-
Momose Haruo
Department Of Biological Science And Technology Tokyo University Of Science
-
Momose Haruo
Department Of Biological Science And Technology Science University Of Tokyo
-
Taguchi S
School Of Agriculture Meiji University:department Of Biological Science And Technology Tokyo Univers
-
Taguchi Seiichi
Department Of Biological Science And Technology Science University Of Tokyo
-
Taguchi S
Department Of Biological Science And Technology Science University Of Tokyo
-
Naoi Yumiko
Department of Biological Science and Technology, Science University of Tokyo
-
Naoi Yumiko
Department Of Biological Science And Technology Science University Of Tokyo
関連論文
- Photovoltaic Effect in Schottky Junction of Poly(3-alkylthiophene)/Al with Various Alkyl Chain Lengths and Regioregularities
- Photocarrier Transport in Regioregular Poly (3-octadecylthiophene) : Optical Propertles of Condensed Matter
- Cationic Polymerization of Styrene with N-Benzyl Pyridinium Salt. Isolation of Polymeric Cationic Species and Its Application to Block Copolymer Synthesis
- Hydrazinium Salts as Novel Thermally Latent Bronsted Acid-Inducing Initiators
- Alkyl Chain Length Dependence of Field-Effect Mobilities in Regioregular Poly(3-Alkylthiophene)Films
- Dependencies of Field Effect Mobility on Regioregularity and Side Chain Length in Poly (Alkylthiophene) Films (Special Issue on Organic Molecular Electronics for the 21st Century)
- Regioregularity vs Regiorandomness : Effect on Photocarrier Transport in Poly(3-hexylthiophene)
- NTAKα and β Isoforms Stimulate Breast Tumor Cell Growth by Means of Different Receptor Combinations
- Syntheses and Polymerizations of (Meth)acrylates Having Containing a Pendant Spiro Ortho Ester and Carbonate Moieties
- Assessment of Plasma Protein S Functional Activity and Antigen in Patients with Liver Cirrhosis
- Effective β-Galactosidase Production by Recombinant Escherichia coli Based on Culture Phase Identification Using Fuzzy Set Theory
- Data Base Simulation of Batch and Fed-Batch Cultures for α-Amylase Production Using a Culture Data Base and a Statistical Procedure
- Identification of a Structural Gene Encoding a Metallothionein-like Domain that Includes a Putative Regulator Protein for Streptomyces Protease Gene Expression
- A Gene Homologous to the Streptomyces Chymotrypsin-like Protease (SAM-P20) Gene Is Tandemly Located
- Photoluminescence from the Even-Parity State in Polythiophene Derivative
- Molecular Cloning of a Partial cDNA Clone Encoding the C Terminal Region of Chicken Breast Muscle Connectin
- Constitutional Isomerism IV. Synthesis and Characterization of Poly(amide-ester)s From Isophthaloyl Chloride and 4-Aminophenethyl Alcohol
- Production and Characterization of Poly(3-hydroxybutyric acid-co-3-hydroxyvaleric acid) from L-Valine by Ralstonia eutropha
- Palladium(0)-Catalyzed Synthesis of Unsaturated Polyesters from Bifunctional Vinyloxirane and Diacids
- Temporal and Spatial Expression of Distinct Troponin T Genes in Embryonic/Larval Tail Striated Muscle and Adult Body Wall Smooth Muscle of Ascidian
- Endogenous Meltrin α Is Ubiquitously Expressed and Associated with the Plasma Membrane but Exogenous Meltrin α Is Retained in the Endoplasmic Reticulum^1
- Optimization of the Microbial Production of a Protein (SSI) Using an Indicator Host-Vector System
- Overexpression and Purification of Microbial Pro-Transglutaminase from Streptomyces cinnamoneum and in vitro Processing by Streptomyces albogriseolus Proteases(ENZYMOLOGY, PROTEIN ENGINEERING, AND ENZYME TECHNOLOGY)
- High Frequency of SSI-Like Protease Inhibitors among Streptomyces
- Novel Thermosetting System Based on Acetylene-containing Polymers Obtained by the Radical Polymerization of Aliphatic Enynes
- Selective Radical Vinyl Polymerization of 4-Phenyl-1-buten-3-yne: Synthesis of a Novel Acetylene-Containing Polymer
- Determination of the Complete Nucleotide Sequence of Brevibacterium lactofermentum Plasmid pAM330 and Analysis of Its Genetic Information(Biological Chemistry)
- A Cold-Adapted Protease Engineered by Experimental Evolution System
- A Subtilisin Inhibitor Produced by Streptomyces bikiniensis Possesses a Glutamine Residue at Reactive Site P1
- Classification of Lactobacillus helveticus Strains by Immunological Differences in Extracellular Proteinases
- EFFECTS OF TEMPERATURE ON THE PHOTOSYNTHESIS OF THE ANTARCTIC GREEN ALGA, CHLORELLA VULGARIS (ABSTRACT) (15th Symposium on Polar Biology)
- EFFECTS OF TEMPERATURE ON THE PHOTOSYNTHESIS OF ANTARCTIC FRESHWATER GREEN ALGAE (ABSTRACT) (14th Symposium on Polar Biology)
- Culture of green algae isolated from fresh-water areas in the Antarctic (abstract)
- Hybrid Simulation of Microbial Behavior Combining a Statistical Procedure and Fuzzy Identification of Culture Phases
- Substrate Specificity Analysis of Microbial Transglutaminase Using Proteinaceous Protease Inhibitors As Natural Model Substrates^1
- An Endogenous Target Protease, SAM-P26, of Streptomyces Protease Inhibitor(SSI): Primary Structure, Enzymatic Characterizaion, and Its Interaction with SSI
- Molecular Cloning and Sequence Analysis of a Gene Encoding an Extracellular Serine Protease from Streptomyces lividans 66
- Polymerizable Anionic Gemini Surfactants: Physicochemical Properties in Aqueous Solution and Polymerization Behavior
- Dedifferentiation and regeneration of skeletal muscle : Their regulatory mechanisms(Symposium on Current Research for Skeletal Muscle)
- Production of Antibacterial Peptide 'Apidaecin Using' the Secretory Expression System of Streptomyces
- Functional Mapping against Escherichia coli for the Broad-Spectrum Antimicrobial Peptide, Thanatin, Based on an In Vivo Monitoring Assay System^1
- Palladium(0)-catalyzed Synthesis of Unsaturated Polyethers from Bifunctional Vinyloxiranes and Bisphenol Analogues
- Communication Between Protease and Protease Inhibitor in the Streptomyces World
- Anionic Ring-Opening Polymerization of Cyclic Thiocarbonates Containing Norbornene and Norbornane Groups Undergoing Volume Expansion on Polymerization
- Fixation of Carbon Dioxide into Polysilsesquioxane Containing Glycidyl Groups
- First Example of Cationic Ring-Opening Polymerization of γ-Thionobutyrolactone
- Partial alteration of a protein Streptomyces subtilisin inhibitor by site-directed mutagenesis.
- Photovoltaic Effect in Schottky Junction of Poly(3-alkylthiophene)/Al with Various Alkyl Chain Lengths and Regioregularities
- Photocarrier Transport in Regioregular Poly (3-octadecylthiophene) : Optical Propertles of Condensed Matter
- NTAK.ALPHA. and .BETA. Isoforms Stimulate Breast Tumor Cell Growth by Means of Different Receptor Combinations.