Syntheses of Recombinant Yellowtail and Flounder Growth Hormones in Escherichia coli
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概要
- 論文の詳細を見る
For syntheses of recombinant yellowtail and flounder growth hormones (r-yGH and r-fGH) in E.coli, expression plasmids were constructed. The expression level of r-yGH and r-fGH in the host cells were very high, reaching 15 and 8% of the total protein, respectively. These product proteins were accumulated in inclusion bodies in the cells. The recombinant hormones were isolated from the pellets in a glutathione reduction/oxidation buffer. The refolded hormones were further purified by DEAE-Toyopearl 650M chromatography to homogeneity. The purified r-yGH and r-fGH were composed of 188 and 174 amino acid residues, respectively, having amino-terminal sequences starting with methionine. The recombinant hormones had potent growth-promoting activities on juvenile rainbow trout Salmo gairdneri in a dose-dependent manner.
- 社団法人日本農芸化学会の論文
- 1992-07-23
著者
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TANAKA MINORU
Department of Thoracic Surgery, Nagoya University School of Medicine
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Ohara Eiji
Enzymological Research Laboratory Nippon Gene Co. Ltd.
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NAKASHIMA KUNIO
Department of Biochemistry, Mie University School of Medicine
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Nakashima Kunio
Department Of Biochemistry Faculty Of Medicine Mie University
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Yamakawa Minoru
Department Of Biochemistry Mie University School Of Medicine:(present Address) Laboratory Of Biologi
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Shoji Kazuaki
Enzymological Research Laboratory Nippon Gene Co. Ltd.
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WATAHIKI Masanori
Department of Bacteriology, Toyama Institute of Health
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TSUDA Momoe
Enzymological Research Laboratory, Nippon Gene Co., Ltd.
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MASUJI Akiko
Department of Biochemistry, Mie University School of Medicine
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USHIRO Hiroshi
Department of Biochemistry, Mie University School of Medicine
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YONEDA Yuko
Enzymological Research Laboratory, Nippon Gene Co., Ltd.
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Tsuda Momoe
Enzymological Research Laboratory Nippon Gene Co. Ltd.
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Yoneda Yuko
Enzymological Research Laboratory Nippon Gene Co. Ltd.
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Masuji Akiko
Department Of Biochemistry Mie University School Of Medicine
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Ushiro Hiroshi
Department Of Biochemistry Mie University School Of Medicine
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Watahiki Masanori
Department Of Biochemistry Mie University School Of Medicine:enzymological Research Laboratory Nippo
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Watahiki Masanori
Department Of Bacteriology Toyama Institute Of Health
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Tanaka Minoru
Department Of Thoracic Surgery Nagoya University School Of Medicine
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Tanaka Minoru
Department Of Anatomy Kyoto Prefectural University Of Medicine
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WATAHIKI Masanori
Department of Agricultural Chemistry, Ibaraki University
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