Conformational Change in a Single Molecular Species, β_3,of β-Conglycinin in Acidic Ethanol Solution
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概要
- 論文の詳細を見る
Several physicochemical experiments were done to obtain further information on the conformational changes occurring in β-conglycinin in acidic-ethanol solution, using a single molecular species of this protein, β_3. By far-UV circular dichroism (CD), a transition fromβ-sheet to α-helical structure was observed upon addition of acidic-ethanol, and the α-helix content was found to reach 76% in 70% ethanol (pH 2). From analyses of near-UV CD and difference absorption spectra, it was found that the tertiary structure of theβ_3 species was significantly altered at ethanol concentrations between 10 and 20%. The profiles of binding of 1-anilinonaphthalene-8-sulfonic acid to theβ_3 species during acidic-ethanol denaturation were indicative of the existence of intermediate conformers in the molten globule-like denaturation state. By measuring Fourier transform infrared spectra and estimating the Stokes radius by dynamic light scattering , theβ_3 molecules were found to aggregate with an increase in ethanol concentration.
- 社団法人日本農芸化学会の論文
- 2001-02-23
著者
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Morita Shimpei
Novelty Materials Research Institute Tsukuba R&d Center Fuji Oil Co. Ltd.
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Tsumura K
Department Of Bioresources Chemistry Faculty Of Agriculture Okayama University
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Kugimiya Wataru
Novelty Materials Research Institute Tsukuba R&d Center Fuji Oil Co. Ltd.
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Kugimiya Wataru
Novelty Materials Research Institute Tsukuba R & D Center Fuji Oil Co. Ltd.
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Tsumura Kazunobu
Novelty Materials Research Institute Tsukuba R & D Center Fuji Oil Co. Ltd.
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Kuwada Masahiro
Department Of Biochemistry Kitasato University School Of Medicine
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ENATSU Makoto
Novelty Materials Research Institute, Tsukuba R&D Center, Fuji Oil Co., Ltd.
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KURAMORI Koichi
Novelty Materials Research Institute, Tsukuba R&D Center, Fuji Oil Co., Ltd.
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SHIMURA Yuki
Department of Biophysical Chemistry, Kitasato University School of medicine
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HASUMI Hideyo
Department of Biophysical Chemistry, Kitasato University School of medicine
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Shimura Yuki
Department Of Biophysical Chemistry Kitasato University School Of Medicine
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Hasumi Hideyo
Department Of Biophysical Chemistry Kitasato University School Of Medicine
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Enatsu Makoto
Novelty Materials Research Institute Tsukuba R&d Center Fuji Oil Co. Ltd.
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Kuramori Koichi
Novelty Materials Research Institute Tsukuba R&d Center Fuji Oil Co. Ltd.
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