Phylogenetic Analysis of Methanogens in Sheep Rumen Ecosystem and Detection of Methanomicrobium mobile by Fluorescence In Situ Hybridization
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概要
- 論文の詳細を見る
The population of methanogens in the sheep rumen microbial ecosystem was studied by using 16S rDNA cloning analysis, epifluorescence microscopy (which detects autofluorescence of a specific cofactor F_<420> in methanogens) and the 16S rRNA-targeted in situ hybridization technique. The 16S rDNA clone libraries were constructed by PCR amplification with an Archaea-specific primer set and partial sequencing of the clonal 16S rDNAs was done. Phylogenetic analysis indicated that the clones were affiliated with Methanomicrobium mobile, Methanobrevibacter ruminantium and Methanobrevibacter smithii. Epifluorescence microscopy (F_<420> autofluorescence) and in situ hybridization by using a newly designed M. mobile-specific 16S rRNA-targeted oligonucleotide probe found that methanogens accounted for approximately 3.6% of total ruminal microorganisms and approximately 54% of the total methanogens were M. mobile.
- 社団法人日本農芸化学会の論文
- 2000-08-23
著者
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NAKAMURA Yutaka
Department of Industrial and Systems Engineering, Setsunan University
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Minato Hajime
Department Of Animal Sciences Ibaraki University
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Kamagata Yoichi
National Institute For Bioscience And Human-technology Aist Miti
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Kawaharasaki Mamoru
National Institue Of Bioscience And Human-technology Agency Of Industrial Science And Technology
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Kawaharasaki Mamoru
National Institute Of Bioscience And Human Technology Agency Of Industrial Science And Technology
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YANAGITA Kazuhiro
National Institute of Bioscience and Human Technology, Agency of Industrial Science and Technology
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SUZUKI Toshihiko
National Institute of Bioscience and Human Technology, Agency of Industrial Science and Technology
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Yanagita Kazuhiro
National Institute Of Bioscience And Human Technology Agency Of Industrial Science And Technology:de
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Suzuki Toshihiko
National Institute Of Bioscience And Human Technology Agency Of Industrial Science And Technology
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Nakamura Yutaka
Department Of Industrial And Systems Engineering Setsunan University
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Nakamura Yutaka
Department Of Animal Sciences Ibaraki University
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