Cloning and Expression of the ccpA Gene Encoding Catabolite Control Protein from Thermoactinomyces sp. E79

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概要

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• The gene ccpA encoding the catabolite control protein A (CcpA) of Thermoactinomyces sp. E79 has been cloned and characterized. Nucleotide sequence analysis of the CcpA clone showed that the cloned fragment contained the full structural gene for a protein of 346 amino acids. The predicted amino acid sequence shows similarity to the transcriptional regulators of the LacIGalR family ; a highly conserved helix-turn-helix motif, which might bind to DNA, was identified through comparison with regulator proteins in this family. The highest sequence identity was obtained when it was compared with the CcpA of Bacillus subtilis (60%) or Bacillus megaterium (60%). The expression of ccpA in Thermoactinomyces sp. E79 was dependent on glucose, which is contrast to the cases of B. subtilis, B. megaterium and S. xylosus. The complementation experiment with the B. megaterium ccpA mutant indicated that the cloned gene was a ccpA.

• 社団法人日本農芸化学会の論文
• 2000-10-23

著者

• Park Sun-young

  韓国

• Oh T‐k

  Korea Res. Inst. Biosci. And Biotechnol. Daejon Kor

• Kim H‐k

  Hanseo Univ. Chungnam Kor

• Kim H‐k

  Hanseo Univ. Seosan Kor

• Lee J‐k

  Korea Res. Inst. Biosci. And Biotechnol. Taejon Kor

• Oh Tae-kwang

  Microbial Enzyme Ru Korea Research Institute Of Bioscience & Biotechnology (kkibb)

• Oh Tae-kwang

  韓国生物科学生物工学研究所細菌酵素研究部門

• Park S‐y

  College Of Pharmacy Kyung Hee University

• Park S‐y

  Korea Res. Inst. Biosci. And Biotechnol. Taejon Kor

• Park Sun-young

  College Of Pharmacy Kyung-hee University

• MOON MinWoo

  Environmental Bioresources Laboratory, Korea Research Institute of Bioscience and Biotechnology

• PARK SunYang

  Environmental Bioresources Laboratory, Korea Research Institute of Bioscience and Biotechnology

• KIM HyungKwoun

  Environmental Bioresources Laboratory, Korea Research Institute of Bioscience and Biotechnology

• YUM DoYoung

  Environmental Bioresources Laboratory, Korea Research Institute of Bioscience and Biotechnology

• KWAK HahnShik

  Department of Genetic Engineering, Paichai University

• OH TaeKwang

  Environmental Bioresources Laboratory, Korea Research Institute of Bioscience and Biotechnology

• LEE JungKee

  Environmental Bioresources Laboratory, Korea Research Institute of Bioscience and Biotechnology

• Moon Minwoo

  Environmental Bioresources Laboratory Korea Research Institute Of Bioscience And Biotechnology:(pres

• Yum Doyoung

  Environmental Bioresources Laboratory Korea Research Institute Of Bioscience And Biotechnology:(pres

• Kwak H‐s

  Sejong Univ. Seoul Kor

• Kim Hyung-kwoun

  Environmental Bioresources Laboratory Korea Research Institute Of Bioscience And Biotechnology:(pres

• Moon Min--Woo

  Environmental Bioresources Laboratory, Korea Research Institute of Bioscience and Biotechnology

• Park Sun-Yang

  Environmental Bioresources Laboratory, Korea Research Institute of Bioscience and Biotechnology

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