High-level Expression of a Recombinant Thermostable Phytase in Bacillus subtilis

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概要

• 論文の詳細を見る

• An efficient expression system was developed in Bacillus subtilis for the large scale production of phytase. The phytase gene with a native promoter derived from Bacillus amyloliquefaciens was cloned in the Bacillus expression vector pJH27 under a strong BJ27 promoter and its expression was optimized. The expression of the phytase gene occurred during late exponential growth and the extracellular phytase production was 2.0 units/ml, which constituted over 90% of the total protein. The yield was 100-fold higher than wild type Bacillus amyloliquefaciens DS11.

• 社団法人日本農芸化学会の論文
• 1999-12-23

著者

• Oh B‐c

  Korea Res. Inst. Biosci. And Biotechnol. Daejon Kor

• Oh T‐k

  Korea Res. Inst. Biosci. And Biotechnol. Daejon Kor

• LEE Jung-Kee

  Microbial Enzyme RU Korea Research Institute of Bioscience & Biotechnology (KRIBB)

• OH Tae-Kwang

  Microbial Enzyme RU Korea Research Institute of Bioscience & Biotechnology (KRIBB)

• Lee J‐k

  Korea Res. Inst. Biosci. And Biotechnol. Taejon Kor

• Kim Young-ok

  Microbial Enzyme Ru Korea Research Institute Of Bioscience & Biotechnology (kkibb)

• Oh Tae-kwang

  Microbial Enzyme Ru Korea Research Institute Of Bioscience & Biotechnology (kkibb)

• Oh Tae-kwang

  韓国生物科学生物工学研究所細菌酵素研究部門

• OH Byung-Chul

  Microbial enzyme RU, Korea Research Institute of Bioscience & Biotechnology (KKIBB)

• Oh Byung-chul

  Microbial Enzyme Ru Korea Research Institute Of Bioscience & Biotechnology (kkibb)

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