Characteristics of Transposon Insertion Mutants of Mandelic Acid-utilizing Pseudomonas putida Strain A10L
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概要
- 論文の詳細を見る
A soil isolate, Pseudomonas putida strain A10L that utilizes mandelate via the mandelate pathway was mutagenized by transposon Tn5-Mob insertion and a mutant 168 lacking mandelate racemase (MR) and a mutant 254 lacking benzoylformate decarboxylase (BFDC) were obtained. Expression of (S)-mandelate dehydrogenase (MDH), BFDC, NAD^+-dependent benzaldehyde dehydrogenase (BDH) and NADP^+-dependent BDH in the MR-lacking mutant was not affected by the insertion, and it was inducible similarly to the wild type strain. On the other hand, expression of MR and MDH in the BFDC-lacking mutant was low and constitutive, and NAD^+- and NADP^+-dependent BDHs were produced at a rather high level under non-induced conditions by the mutant. Genes for MR (mdlA), MDH (mdlB), and BFDC (mdlC) were indicated to be organized in an operon in the order of mdlCBA. Optical resolution to obtain (R)-mandelate, a useful synthon for pharmaceuticals, was shown to be performed with the MR-lacking mutant.
- 社団法人日本農芸化学会の論文
- 1996-07-23
著者
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NAKAMURA TOMOYUKI
Department of Orthopedic Surgery, Graduate School of Medical Sciences, Kyushu University
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SHIMAO MASAYUKI
Departments of Biotechnology Faculty of Engineering, Tottori University
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Okuda Akira
Department of Mechanical and Systems Engineering, Faculty of Engineering, Gifu University
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Okuda Akira
Department Of Biotechnology Tottori University
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Nakamura Tomoyuki
Department Of Biotechnology Tottori University
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Nakamura Tomoyuki
Department Of Applied Chemistry Fuculty Of Engineering Naogya University
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HARAYAMA Shigeaki
Department of Medical Biochemistry, University of Geneva
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Harayama S
Marine Biotechnol. Inst. Iwate Jpn
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Shimao Masayuki
Department Of Biotechnology Tottori University
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Shimao Masayuki
Department Of Medical Biochemistry University Of Geneva:department Of Biotechnology Tottori Universi
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Harayama Shigeaki
Department Of Medical Biochemistry University Medical Center
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