Improvement of a Cytidine-producing Mutant of Bacillus subtilis Introducing a Mutation by Homologous Recombination

概要

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Bacillus subtilis No. 428 is a cytidine-producing mutant strain derived from wild-type strain No. 122. To identify the rate-determining factor for production of cytidine by strain No. 428, we examined the properties of the strain. We found that carbamyl phosphate synthetase P (CPSase P) from strain No. 428 was sensitive to feedback inhibition by uridine 5'-monophosphate (UMP), and therefore, this enzyme reaction was the rate-determining step for cytidine production. Thus, we introduced a mutation causing lack of feedback inhibition of CPSase P into the CPSase P-deficient mutant of strain No. 428 by homologous recombination. Some of the transformants had higher productivity of cytidine. Among them, strain No. 515 produced 18. 8 mg/ml cytidine in the culture. CPSase P from strain No. 515 was freed from feedback inhibition by UMP, having obtained the characteristic of the donor.
社団法人日本農芸化学会の論文
1995-11-23