A Convenient Method for Preparation of the Calcium Ion-Binding Protein Annexin V(MEDICAL BIOTECHNOLOGY)
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概要
- 論文の詳細を見る
This study presents a novel method for the production and purification of annexin V using cation exchange chromatography in the presence of calcium ions at neutral or alkaline pH. This method enables the handling of a large quantity of sample at one time without deterioration of the adsorption capacity of the cation exchange carrier by contaminating proteins, as well as the production of the calcium ion-binding protein annexin V with high purity on an industrial scale from both recombinant products and native products.
- 社団法人日本生物工学会の論文
- 2004-02-25
著者
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Soejima Kenji
カナダ
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Soejima Kenji
化学及血清療法研究所
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MAEDA Terunobu
Civil Chemical Engineering Corporation
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SUGAWARA Keishin
Applied Research Department, The Chemo-Sero-Therapeutic Research Institute (KAKETSUKEN)
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Maeda T
Civil Chemical Engineering Corporation
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Maeda Toshihiro
Applied Research Department The Chemo-sero-therapeutic Research Institute (kaketsuken)
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Koyanagi Satoshi
Applied Research Department, The Chemo-Sero-Therapeutic Research Institute (Kaketsuken)
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Mizokami Hiroshi
Applied Research Department, The Chemo-Sero-Therapeutic Research Institute (Kaketsuken)
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FURUKAWA SHINICHI
Applied Research Department, The Chemo-Sero-Therapeutic Research Institute (Kaketsuken)
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ONCHI TATSUFUMI
Applied Research Department, The Chemo-Sero-Therapeutic Research Institute (Kaketsuken)
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SOEJIMA KENJI
Applied Research Department, The Chemo-Sero-Therapeutic Research Institute (Kaketsuken)
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YOSHIZAKI HIDEO
Tokyo New Drug Research Laboratories II, Kowa Company, Ltd.
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HIRAYAMA CHUICHI
Faculty of Engineering, Kumamoto University
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Sugawara Keishin
Biologics Development & Production Department The Chemo-sero-therapeutic Research Institute (kak
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Sugawara Keishin
Applied Research Department The Chemo-sero-therapeutic Research Institute (kaketsuken)
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Koyanagi Satoshi
Biologics Development & Production Department The Chemo-sero-therapeutic Research Institute (kak
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Mizokami Hiroshi
Biologics Development & Production Department The Chemo-sero-therapeutic Research Institute (kak
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Mizokami Hiroshi
Department Of Research And Development
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Furukawa Shinichi
Applied Research Department The Chemo-sero-therapeutic Research Institute (kaketsuken)
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Furukawa Shinichi
Applied Chemistry Department Kyushu Institute Of Technology
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Soejima Kenji
Applied Research Department The Chemo-sero-therapeutic Research Institute (kaketsuken)
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Yoshizaki H
Tokyo New Drug Research Laboratories Ii Kowa Company Ltd.
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FURUKAWA Sachiko
Kizakura Co. Ltd.
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Furukawa S
Kizakura Co. Ltd.
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Onchi Tatsufumi
Applied Research Department The Chemo-sero-therapeutic Research Institute (kaketsuken)
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Yoshizaki Hideo
Tokyo New Drug Research Laboratories Ii Kowa Company Ltd.
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Hirayama C
Faculty Of Engineering Kumamoto University
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Murakami Toshio
Applied Research Department The Chemo-sero-therapeutic Research Institute (kaketsuken)
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