Structure and expression of cDNA for an inhibitor of blood coagulation isolated from human placenta: A new lipocortin-like protein.
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概要
- 論文の詳細を見る
An inhibitor of blood coagulation, a new protein with an apparent molecular weight of 34, 000 and an isoelectric point of 4.9, was purified from human placental tissue by EDTA extraction. Five cDNA clones were isolated from the human placental λgt11 cDNA library using the mouse monoclonal antibody raised against the coagulation inhibitor as the probe. The longest insert consists of 1, 566 nucleotides, and contains 960 nucleotides entirely encoding the 320 amino acids of the inhibitor, and a poly A tail. The deduced amino acid sequence was corroborated by chemical analyses of the protein. The entire amino acid sequence shows homology to those of lipocortin I, lipocortin II, andendonexin-related proteins. The cDNA for the inhibitor was expressed in Escherichia coli under the regulation of the trc promotor of the plasmid pKK233-2. The resulting recombinant protein manifested inhibitory activities against both blood coagulation and phospholipase A2 activity, as did the coagulation inhibitor isolated from human placenta.
- 社団法人 日本生化学会の論文
著者
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Iwasaki Akio
Department Of Work System And Health Institute Of Industrial Ecological Sciences University Of Occup
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Suda Makoto
Department Of Biosignal Engineering Kowa Research Institute
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Miyata Toshiyuki
Department Of Biology Faculty Of Science Kyushu University
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Murata Makoto
Department Of Chemical Science And Engineering Graduate School Of Engineering Science Osaka Universi
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Maki Masahiro
Department Of Obstetrics And Gynecology Akita University School Of Medicine
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Sato Fumiyasu
Tokyo New Drug Research Laboratories I Pharmaceutical Division Kowa Co Ltd
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Shidara Yoshihiro
Department Of Obstetrics And Gynecology Akita Kumiai General Hospital
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Yoshizaki Hideo
Tokyo New Drug Research Laboratories Ii Kowa Company Ltd.
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MIZOGUCHI Toshimi
Tokyo Research Laboratories, Kowa Co., Ltd.,
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Nakao Hiroshi
Department Of Cell Biology Kowa Research Institute
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Hirata Mitsuteru
Tokyo Research Laboratories Kowa Co. Ltd.
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ARAI Koichi
Tokyo Research Laboratories, Kowa Co., Ltd
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SUDA Makoto
Department of Cell Biology, Kowa Research Institute, Kowa Co. Ltd.
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NAGOYA Takao
Department of Cell Biology, Kowa Research Institute, Kowa Co. Ltd.
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SAINO Yushi
Department of Cell Biology, Kowa Research Institute, Kowa Co. Ltd.
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HIRATA Mitsuteru
Tokyo Research Laboratories, Kowa Co., Ltd
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