Effects of Peptide Antigenic Determinant Properties on Adsorption Equilibrium of Anti-Peptide Antibodies
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概要
- 論文の詳細を見る
The antibodies to hydrophilic and hydrophobic peptides representing residues 85-94 of cytochrome c (P1) and 148-158 of tobacco mosaic virus protein (P2), respectively, were produced in rabbits. Peptide-keyhole limpets hemocyanin (KLH) conjugates and peptide-polyvinylpyrrolidone (PVP) mixtures were used for immunization. From the binding experiments of analogous synthetic peptides in immunoadsorbents, the major fractions of all the polyclonal anti-peptide antibodies were found to bind to the antigenic determinants localized in the four to six C-terminal amino acids of the peptides. In both the anti-P1 and anti-P2 antibodies produced by immunizing peptide-KLH conjugates, the C-terminal carboxylic acid group was essential for antibody binding. On the other hand, the effect of pH and ionic stregth on the adsorbed amount of the antibodies depended on the peptide. Thet is, the adsorbed amount of P1 to both the anti-P1 antibodies produced by the different immunization methods decreased significantly with increasing ionic strength, while the adsorbed amount of P2 to the antibodies exhibited a minimum at around ionic strength 1.0. These results indicate that the dependence of antibody affinities on pH and ionic strength is related to the properties of antigenic determinants.
- 公益社団法人日本生物工学会の論文
- 1991-12-25
著者
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Kondo Akihiko
Applied Chemistry Department, Kyusyu Institute of Technology
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Furukawa Shinichi
Applied Chemistry Department Kyushu Institute Of Technology
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Taira Masato
Applied Chemistry Department Kyushu Institute Of Technology
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Higashitani K
Kyushu Inst. Technology Kitakyushu Jpn
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Higashitani Ko
Applied Chemistry Department Kyushu Institute Of Technology
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Kondo Akihiko
Applied Chemistry Department Kyushu Institute Of Technology
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KONDO AKIHIKO
Applied Chemistry Department, Kyushu Institute of Technology
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