Affinity Purification of Antibodies Using Immunomicrospheres

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概要

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• Antigen-immobilized colloidal particles (immunomicrospheres) of submicrometer size were used for the immunoafinity separation of antibodies. Carboxylated latex particles (P(St/AAm)-H) were prepared by copolymerization of styrene (St)/acrylamide (AAm) and by subsequent hydrolysis and were coupled covalently with antigen (bovine serum albumin; BSA) by the carbodiimide method. The anti-BSA antibody binding capacity of the immunomicrospheres increased with increasing amount of immobilized BSA up to the value which corresponds to the high packing density of the antibody molecules on the surface. The binding ratios of the immunomicrospheres, defined as the ratio of mol antibody adsorbed to mol BSA immobilized, were comparative to those of the conventional immunoadsorbents for affinity chromatography and did not decrease during repeated cycles of adsorption and desorption. By use of the immunomicrospheres, the antibodies wee successfully purified from antisera.

• 公益社団法人日本生物工学会の論文
• 1992-10-25

著者

• Kondo Akihiko

  Applied Chemistry Department, Kyusyu Institute of Technology

• Higashitani Ko

  Applied Chemistry Department Kyushu Institute Of Technology

• YAMASAKI RYO

  Applied Chemistry Department, Kyushu Institute of Technology

• Kondo Akihiko

  Applied Chemistry Department Kyushu Institute Of Technology

• Yamasaki Ryo

  Applied Chemistry Department Kyushu Institute Of Technology

関連論文

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• Bioconversions in an Aqueous Two-Phase System Using Enzymes Immobilized on Ultrafine Silica Particles
• Immunologial Agglutination Kinetics of Latex Particles with Covalently Immobilized Antigens
• Affinity Purification of Antibodies Using Immunomicrospheres

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