Bioconversions in an Aqueous Two-Phase System Using Enzymes Immobilized on Ultrafine Silica Particles

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概要

• 論文の詳細を見る

• Bioconversions of corn starch and bovine hemoglobin in an aqueous two-phase system using α-amylase and papain immobilized on ultrafine silica particles (average diameter 15 nm) were studied. Both α-amylase and papain were immobilized onto the ultrafine silica particles with high efficiency by convalent cross-linking with glutaraldehyde, and both enzymes showed high activities. Since these immobilized enzymes were totally partitioned to the polyethylene glycol (PEG)-rich top phase in PEG/dextran aqueous two phase systems, the products were recovered from the bottom phase. These reaction systems were found to be effective for extractive bioconversions.

• 公益社団法人日本生物工学会の論文
• 1994-06-25

著者

• Kondo Akihiko

  Applied Chemistry Department, Kyusyu Institute of Technology

• Higashitani Ko

  (present Address)chemical Engineering Department Kyoto University

• URABE TAKAFUMI

  Applied Chemistry Department, Kyushu Institute of Technology

• Kondo Akihiko

  Applied Chemistry Department Kyushu Institute Of Technology

• Urabe Takafumi

  Applied Chemistry Department Kyushu Institute Of Technology

関連論文

• Spectral Changes of Lysozyme Adsorbed on Ultrafine Silica Particles
• Effects of Peptide Antigenic Determinant Properties on Adsorption Equilibrium of Anti-Peptide Antibodies
• Immunological Agglutination Behavior of Latex Particles with Covalently Immobilized Antibodies
• Bioconversions in an Aqueous Two-Phase System Using Enzymes Immobilized on Ultrafine Silica Particles
• Immunologial Agglutination Kinetics of Latex Particles with Covalently Immobilized Antigens
• Affinity Purification of Antibodies Using Immunomicrospheres

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