Uptake and Utilization of Ectoine by Halotolerant Brevibacterium sp. JCM 6894 Subjected to Osmotic Downshock(MICROBIAL PHYSIOLOGY AND BIOTECHNOLOGY)
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概要
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The concentration changes of the cyclic amino acid ectoine (1,4,5,6-tetrahydro-2-methyl-4-pyridine carboxylic acid) in Brevibacterium sp. JCM 6894 cells subjected to an osmotic downshock were investigated. When the cells grown in the presence of 2 M NaCl were suspended in deionized water, they immediately released about 60% of the ectoine synthesized intracellu larly. During the subsequent incubation, we observed that both the extra- and intracellular concentrations of ectoine were reduced almost linearly with the incubation time. When ectoine was provided externally to the downshocked cells, a similar reduction in both intra- and extracellular ectoine concentrations was recognized. In addition, we observed an increase in ectoine accumulation at about 10 h of incubation, which indicates that ectoine was taken up by such downshocked cells in the absence of external Na^+. Furthermore, the downshocked cells showed higher levels of survival, respiration, and growth in the presence of ectoine than in its absence. The ability to take ectoine up was induced in the cells grown in the presence of>0.25 M NaCl for>12 h. Thus, we conclude that even under the lower osmotic condition ectoine might be taken up and subsequently utilized by strain JCM 6894 subjected to the osmotic downshock, indicating that the uptake of ectoine by such cells occurred for the survival and growth of the cell itself rather than for cellular osmoregulation.
- 社団法人日本生物工学会の論文
- 2002-11-25
著者
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永田 進一
Environmental Biochemistry Group Research Center For Inland Seas Kobe University
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NAGATA SHINICHI
Research Center for Inland Seas, Kobe University
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Wang Y
神戸商船大学船貨輸送研究施設
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IKEUCHI TOMOHIKO
Research Institute for Marine Cargo Transportation, Kobe University of Mercantile Marine
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Jin Yi
神戸商船大学船貨輸送研究施設
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WANG YAO
Research Institute for Marine Cargo Transportation, Kobe University of Mercantile Marine
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JIN YI
Research Institute for Marine Cargo Transportation, Kobe University of Mercantile Marine
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Ikeuchi T
Research Institute For Marine Cargo Transportation Kobe University Of Mercantile Marine
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Wang Yao
Research Institute For Marine Cargo Transportation Kobe University Of Mercantile Marine
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Ikeuchi Tomohiko
Research Institute For Marine Cargo Transportation Kobe University Of Mercantile Marine
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Nagata Shinichi
Research Center For Inland Seas Kobe University
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