A Calcium Antagonist Protects against Doxorubicin-Induced Impairment of Calcium Handling in Neonatal Rat Cardiac Myocytes
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概要
- 論文の詳細を見る
The effects of doxorubicin(DOX) on intracellular calcium transients and the cardioprotective effects of a calcium antagonist on DOX-induced impairment of calcium handling were examined in neonatal rat cultured cardiac myocytes. Cultured cardiac myocytes isolated from neonatal Wistar-Kyoto rats were treated with DOX for 24h. Field-stimulated calcium transients in single myocytes were measured in the presence or absence of isoproterenol using fura-2/AM. Calcium transients were also measured after the addition of DOX to myocytes pretreated with a calcium antagonist, benidipine. DOX reduced the amplitude, maximum velocity of increase and decrease of calcium transients and prolonged the time course of calcium transients and impaired the β-adrenoceptor responsiveness of calcium transients in a concentration-dependent manner. The DOX-induced impairment of calcium transients and β-adrenoceptor responsiveness was improved by 10^<-8>mol/L of benidipine. However, these improvements decreased with increasing concentrations of benidipine. DOX impaired both the mobilization and removal of intracellular calcium ions in contraction-relaxation cycles and the response of calcium transients to β-adrenoceptor stimulation. Appropriate concentration of benidipine ameliorated DOX-induced impairment of calcium dynamics, suggesting that benidipine, a long-acting calcium antagonist, has potential clinical usefulness on DOX-induced abnormal calcium handling.
- 社団法人日本循環器学会の論文
- 1999-01-20
著者
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HONDA MASAAKI
The 4th Department of Internal Medicine, Shimane Medical University
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Honda Masaaki
The 4th Department Of Internal Medicine Shimane Medical University
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Takabatake Toshikazu
The 4th Department Of Internal Medicine Shimane Medical University
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Takabatake Toshikazu
Fourth Department Of Internal Medicine Shimane Medical University
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Kuramochi Takehiko
The 4th Department of Internal Medicine, Shimane Medical University
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Maeda Atsuyoshi
Fourth Department of Internal Medicine, Shimane Medical University
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Honda Masaaki
Fourth Department of Internal Medicine, Shimane Medical University
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Kuramochi Takehiko
Fourth Department of Internal Medicine, Shimane Medical University
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Maeda Atsuyoshi
Fourth Department Of Internal Medicine Shimane Medical University
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Kuramochi Takehiko
The 4th Department Of Internal Medicine Shimane Medical University
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