EFFECT OF CYCLIC GMP AND SULFHYDRYL ON PROSTACYCLIN PRODUCTION BY HUMAN VASCULAR ENDOTHELIAL CELLS

概要

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The relationship between sulthydryls and cGMP has been observed in several biological processes. Captopril is a sulfhydryl-containing angiotensin converting enzyme (ACE) inhibitor, that decreases PGI_2 production in cultured human vascular endothelial cells. Enalapril does not appear to have this property. The role of cyclic GMP (cGMP) and sulthydryls in the regulatory mechanisms in captopril-induced PGI_2 production and Ca^<++> kinetics was investigated. Brady-kinin and Ca ionophore A23187 enhanced PGI_2 production, and increased the cytosolic free Ca^<++> concentration ([Ca^<++gtn]_i). It was observed that 8-bromo cGMP increased intracellular cGMP concentration ([Ca^<++>]_i), and decreased PGI_2 production without changing [Ca^<++>]_i. Sulthydryl containing compounds such as captopril. N-acetylcysteine and glutathione decreased PGI_2 production via increased [cGMP]_i. Enalapril, an ACE inhibitor without sulthydryls, has no effect on PGI_2 production, [Ca^<++gtn]_i and [cGMP]_i. These results suggested that the presence of sulfhydryl groups is an important factor in the ability of vasoactive substances to induce PGI_2 production.
社団法人日本循環器学会の論文
1991-06-20