Changes in Renalα_2-Adrenoceptor in Experimental Hypertension in Rats : The 12th Conference on the Pathogenesis of Hypertension

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α_2-Adrenoceptors were studied in renal membrane fractions from spontaneously hypertensive (SHR), two-kidney, one clip hypertensive (2K, 1C HT) and DOCA-salt hypertensive (DOCA-salt HT) rats, using radioligand binding method. α_2-Adrenoceptor concentration in the kidney measured by [^3H] yohimbine binding was significantly increased in SHR at 4 weeks old (41.5±2.8 fmol/mg protein, mean ±SEM, p<0.01), 12 weeks old (54.9±2.5 fmol/mg protein, p<0.01) and 35 weeks old (59.8±3.4 fmol/mg protein, p<0.01) as compared with age-matched Wistar-Kyoto rats (WKY, 31.5±2.5, 40.9±1.8, 47.8±2.0 fmol/mg protein, respectively). There were no significant differences in binding affinity and 5'-nucleotidase activity (plasma membrane marker enzyme) between SHR and WKY at any age. In 2K, 1C HT rats, α_2-Adrenoceptor concentration in the clipped kidney was higher than that of control rats, but α_2-Adrenoceptor concentration in the unclipped kidney was unchanged. binding affinity and 5'-nucleotidase activity showed no significant changes in renal hypertensive rats. In DOCA-salt HT rats, no significant change was found in concentration and affinity of renal α_2-Adrenoceptor. The observed increase in renal α_2-Adrenoceptor concentration in SHR may contribute to the pathogenesis and maintenance of hypertension through increased sodium and water reabsorption in the kidney.
社団法人日本循環器学会の論文
1983-10-20