F-0936 単一細胞を対象とした微小水圧制御によるマイクロマニピュレーションシステムの開発(G02-4 細胞)(G02 バイオエンジニアリング部門一般講演)

概要

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In the Genetic Engineering for the Single-Cell, it is hard for experimenter to manipulate Single-Cell (protoplast) to the objective place many times for the purpose of making a physical or chemical stress to each protoplast after manipulation. To attain this purpose, realizing accurate manipulation and find a new means to discriminating cell is necessary. We solve these problems the following ways. (1) In the process of Single-Cell manipulation, it is quite important to treat the cell as softly as possible to minimize the effect of cell manipulation or the cell viability. For this purpose, we developed manipulation system by controlling of water pressure. The system enabled soft touch vacuum and accurate release. (2) We made a Micro-Well, which consist of 16-φ0.1 holes, to put Single-Cell into each holes.
一般社団法人日本機械学会の論文
2001-08-22