2.全身性エリテマトーデスの抗核抗体と補体(第3回補体シンポジウム III 補体とその関連領域)

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1) The sera of patients suffering from S.L.E. contain a series of substances which react upon components of nuclei. A new and simple method for the detection of complement-fixing antinucleoprotein antibody was devised by using conglutination, and compared with the other methods for detection of antinuclear antibodies. The procedure of this method (L.E. conglutination test) was to and 0.01 ml of Latex-Nucleoprotein reagent (Hyland) and 0.2 ml of fresh bovine serum in the S.L.E. serum or its diluents. The tubes were incubated for 60 minutes at 37℃ in waterbath with continuous shaking. After incubation the resultant visible agglutination was read with the naked eye. Comparison of the L.E. conglutination test with fluorescent antibody technique, L.E. cell test and L.E. test (Hyland) were made. The L.E. conglutination test was positive 26 of 58 samples of S.L.E(44.8%), and the tier of its test was well correlated with clinical activity of S.L.E. but not so sensitive as fluorescent antibody technique or L.E. cell test. 2) Since the discovery of the L.E. cell phenomenon, it is well known that the four fcators are concerned in the formation of L.E. cell, ie. L.E. cell factor, damaged cell nuclei, leucocyte and complement. Several experiments have shown that in the first phase L.E. cell factor in the S.L.E. serum gains contact with damaged leucocyte cell nuclei in a short time. When heparin, Na-EDTA or Mg-EDTA had been added previously to the blood of S.L.E., L.E. cell could not be found or was much decreased. This fact suggests that the complement is necessary in the second phase of phagocytosis of free hematoxylin body by leucocytes. Then in order to determine what components of complement are necessary in the L.E. cell formation, following experiments were made. In the L.E. cell test by the blood clot technique, Saldox, Phrolizine or Cu-Chlorophyllin were added previously to the blood, and the number of L.E. cell were compared semiquantitatively with the standard method. By the addition of Saldox or Phlorizine to the blood, L.E. cells were decreased very much, but in cases of Cu-Chlophyllin containing blood the L.E. cell formation was almost never influenced. From these results, it may be concluded that the components of serum complement necessary for the L.E. cell formation are C' 1423c, and that these components of C'accelerate phagocytosis of specifically sensitized nuclei by leucocytes.
日本アレルギー学会の論文
1966-11-30

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