62 トマト ACC 合成酵素 (LE-ACS2) のリン酸化と ETO1 ホモログによる翻訳後制御機構の解析

概要

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ACC synthase (ACS), which is a key enzyme in the pathway of ethylene biosynthesis, is regulated transcriptionally. However, recent studies have raised the possibility that ACS is regulated not only transcriptionally but also post-translationally. We found that LE-ACS2,which is tomato wound-inducible isozyme, was phosphorylated on serine-460 residue and the phosphorylation was involved in turnover of LE-ACS2. On the other hand, the candidate of ACS binding protein that binds the phosphorylation site of ACS is though to be ETO1 homologue, which is the product of the gene that causes eto1 (ethylene over producer 1) mutant of Arabidopsis. Therefore, we tried to identify the tomato ETO1 homologue and to analyze the binding to LE-ACS2. As a result, we cloned the tomato ETO1 homologue, LE-EOL1 (ETO1-LIKE), with RT-PCR fragments that amplified by primer based on conserved amino acid sequences of ETO1 related proteins. LE-EOL1 cDNA consists of 2,930 bp and encodes 886 amino acids. The primary structure of LE-EOL1 reveals one BTB/POZ domain and four TPR domains. The in vitro binding assay showed that LE-ETO1 bound LE-ACS2.
植物化学調節学会の論文
2002-10-31