42 頂芽切除後の第2節で発現するIPT(isopentenyltransferase)の解析

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Apical dominance is a phenomenon in which the terminal bud inhibits the outgrowth of axillary buds. The most widely accepted explanation of the mechanism of apical dominance is that auxin inhibits the growth of axillary buds, whereas cytokinin promotes the growth of axillary buds. It is thought that auxin acts to control the concentration of cytokinin derived from the roots. However, little is known about the molecular mechanism of dormancy in axillary buds. Auxin that is transported basipetally in stem from terminal buds, is not applied to axillary buds is required to inhibit outgrowth of the axillary buds. Based on this observation, we analyzed the genes that expressed in stem, especially node, before and after decapitation. We constructed the subtracted cDNA libraries from the second nod mRNAs before decapitation as tester and ones 3 hr atier decapitation as driver in pea (Pisum sativum L. CV. Alaska) seedlings, and vice versa. As the results from sequencing of more than 500 clones, we found that in node before decapitation auxin inducible genes including AUX/IAA families were expressed. On the other hand, in node after decapitation, adenylate isopentenyltransferase (IPT), which is thought to be a key enzyme of cytokinin biosynthesis, was expressed, implying that cytokinin was biosynthesized at node, not in roots after decapitation, and strongly suggesting that expression of some IPT was repressed by auxin. This result supported that cytokinin is naturally initiator of out growth of the axillary buds. We are investigating the expression patterns of these genes.
植物化学調節学会の論文
2001-10-09

42 頂芽切除後の第2節で発現するIPT(isopentenyltransferase)の解析

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