モデルマウスを用いた漢方方剤の利水作用の検証

概要

論文の詳細を見る
The regulatory effects of a single administration of 8 kinds of extract powders of Kampo formulations (Gorei-san, Sairei-to, Chorei-to, Boi-ogi-to, Toki-shakuyaku-san, Eppi-ka-jutsu-to, Ryokei-jukkan-to or Gosha-jinki-gan, 300 mg/kg, p.o.) on body fluid were examined in two types of water-deprived and -overloaded mice, by determining the urinary volume and body weight for 4 h after administration of each drug. The water-overloaded mice were produced by a single intraperitoneal pretreatment of desmopressin acetate followed by an injection of physiological saline (90 ml/kg, i.p.) . Furosemide administered orally (5 mg/kg) significantly increased the urinary volume in both types of model mice almost equally. On the other hand, the administration of Gorei-san or Sairei-to showed significant increase of urinary volume in water-overloaded mice, while there was no change in urinary volume after its administration to water-deprived mice. The urinary volume tended to be increased by the administration of Chorei-to, Boi-ogi-to, Toki-shakuyaku-san, Eppi-ka-jutsu-to or Ryokei-jukkan-to only in water-overloaded mice. The urinary volume after administration of vehicle and Gosha-jinki-gan was not significantly different in both water-deprived and -overloaded mice. It was suggested that a pair of water-deprived and -overloaded model mice in this study could be a valuable tool for further studies on the regulatory effects of Kampo formulations on body fluid.
2000-06-30