LC/ESI-MSを用いたスギ花芽に含まれる内生ジベレリンの分析

概要

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Gas chromatography- mass spectrometry (GC/MS) is being used for the same identification of the endogenous plant hormone and the quantitative analysis frequently. However, complicated purification and erivatization are necessary for the GC/MS analysis of the endogenous plant hormone. We have examined the way of analyzing it which high Performance liquid chromatography-mass spectrometry (LC/MS) was used for the purpose of the simplification of the same identification of the endogenous plant hormone and the quantitative analysis and a small quantity hiqh sensitivity-ization. So, it considered whether LC/ES-MS could be applied to a small quantity high sensitivity analysis of gibberellins (Gas) here. It detected that the fragmentation which was characteristic of each gibberellins were recognized by doing the MS/MS analysis of the full scanning mode with all gibberellins used for this analysis. It became clear with all GAs used for the analysis that a fixed quantity was possible as that result. Gibberellin A_1, GA_3, GA_4, GA_5,GA_9, GA_<12>, GA_<15>, GA_<20> were identified from the male f1ower and GA_1, GA_3, GA_5, GA_<20> were identified from the bisexual flower. The possibilities that it could cope with a small quantity high sensitivity analysis of GAs were shown in LC/ESI-MS by these things.
植物化学調節学会の論文
2000-11-02