カボチャのジベレリン20-oxidase遺伝子の導入によるわい化トレニアの作出と解析

概要

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We produced dwarf Torenia plants by genetic engeneering using gibberellin (GA) 20-oxidase gene from pumpkin immature seeds. A strong promoter cassette (El2-Ω) was used to facilitate the 20-oxidase expression in transgenic plants. Eighteen transformants were obtained by kanamycin selection, three of which (#1-1, 1-6, 1-7) showed a dwarf-phenotype with smaller leaves and refuced hypocotyls and internodes compared with those of wild-type plants. However, no remarkable differences were observed between the normal-type in transgenic plants and wild-type plants. Transgene was detected by PCR in all transgenic lines showed dwarf and normal growth phenotypes. As the result of northern blot analysis, the transcripts for the transgene were detected in 2 lines of dwarf-type (#1-1 and 1-6) and did not in 1 dwarf line (#1-7) and normal-type. Production of dwarf plants would be the results of alteration of GA biosynthetic pathways by overexpression of pumpkin GA 20-oxidase. Furthermore, because endogenous GA biosynthetic enzymes could be regulated in transgenic torenia, GA 20-oxidase and 3β-hydroxylase genes were partially cloned by RT-PCR and 3'-RACE. 20-oxidase genes were homologous more than 65% and 3β-hydroxylase gene was more than 60% of the corresponding Arabidopsis genes, respectively.
植物化学調節学会の論文
2000-11-02