70 アブラナ科植物における自家不和合性の分子機構

概要

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Self-incompatibility in Brassica is genetically controlled by the multiple alleles at a single locus, S. To date, two stigmatically expressed higly polymorphic genes have been found in the S locus. One is an extracellular glycoprotein called the S locus glycoprotein (SLG), and the other is a transmembrane serine/threonine receptor kinase called the S receptor kinase (SRK) whose extracellar domain is similar to SLG. They are considered to be constituting a receptor complex that receives some kind of pollen ligand encodede in the S locus. But the molecular nature of this pollen ligand remains unknown. To identify this pollen ligand gene, we used the technique of fluorescent differential desplay (FDD). The cDNAs were prepared from the anthers of S^8 and S^12 homozygotes of B. rapa. Using these cDNAs as templates FDD analyse were parformed with 240 independent primer combination. We obtained a number of S-haplotype specific genes. Among them, two genes were shown to share several characters with PCP-A1, a pollen coat protein which was known to intract with SLG.
植物化学調節学会の論文
1999-10-01