タラノキ(Aralia elata Seemann)培養体由来根組織からの不定芽,不定胚形成および植物体再生(繁殖・育苗)

概要

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Root segments of plantlets (Aralia elata) grown by in vitro culture, were used as explants and cultured in the dark at 25℃ on MS medium supplemented with growth regulators. About 80-100% of root segments formed calli on MS medium supp6lemented with 0.05-4.0 mg/l 2, 4-dichlorophenoxyacetic acid (2, 4- D). After 30 days of culture, calli formed on MS medium containing 0.05 mg/l 2,4-D, were transferred on hormone-free MS medium and cultured at 25℃ in 16 h light (3,000 lux). Somatic embryogenesis was observed from the calli formed on medium supplemented with more than 0.5 mg/l 2, 4-D. Many somatic embryos and adventitious shoots were induced from the calli and developed into small plantlets by a subculture of 20-30 days interval. After acclimatization on vermiculite, regenerated plantlets were transplanted to a mixture of sandy masa soil / bark compost (3:1, v/v). Nearly all plants were established and grew into nursery stocks after 45 days.
園芸学会の論文
2004-12-15