細胞選抜由来自殖系統ニンジンのAl耐性とそれらの根からのクエン酸放出の部位的変異

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1) We used the Al-sensitive carrot cultivar Kuroda 5 sun and cultured cell selection using Al-phoshate media of different pHs to produce S_1 lines from regenerated plants. 2) We selected Al-tolerant lines by growing in Kawatabi Soil (acidic non-allophanic andosol). From the pH 5.0 selection, we obtained three lines (Nos.63,65,77) which were more Al-tolerant than their original cultivar Kuroda 5 sun. 3) Using a visual detection method for Al tolerance level in the plant root by decoloring filter paper stained by hematoxylin and HPLC analysis, we investigated the exudation of organic acid. Consequently, the citric acid was exuded from the Al-treated roots of these lines. It became clear that there existed three types of local variation in citric acid exudation in the carrot root of young plant : the first is root tip type (No.77), the second is root base type (No.63) and the third is overall root type (No.65). 4) The aluminum tolerance mechanism in carrot is the detoxification of Al by chelating citric acid exuded from the root. In the case the region of citric acid exudation is limited to only one part, there is a possibility of the inhibition of elongation or the expansion of root occuring. These may cause insufficient enlargement of the root or abnormal root growth. Therefore, if the breeding of Al tolerance in a root crop such as carrot is started, it is necessary to make selections for the lines or cultivars that have an Al tolerance mechanism where organic acid is exuded from all areas of the root in response to Al.
社団法人日本土壌肥料学会の論文
2001-02-05

細胞選抜由来自殖系統ニンジンのAl耐性とそれらの根からのクエン酸放出の部位的変異

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