EFFECTIVENESS OF TETRAZOLIUM SALTS IN MICROBIAL ECOLOGICAL STUDIES IN SUBMERGED SOIL
スポンサーリンク
概要
- 論文の詳細を見る
Several trials to obtain information on the living microorganisms at micro-sites of the submerged soil by the novel utilization of the tetrazolium salts were described. Results are summarized as follows : (1) The air-dried soil was incubated with TTC (triphenyl tetrazolium chloride) under submerged condition according to the modified double layer plate method. The red formazan (the reduced product of TTC) was precipitated at the biologically active sites by the dehydrogenase activity. Using a binocular microscope, most of the biologically active sites were found to be localized on and around the organic debris. (2) The amount of nitrogen mineralized by incubating the soil under submerged condition correlated well not only with the amount of formazan formed biologically in the submerged soil but also with the amount of formazan formed non-biologically by heating the soil with TTC in Ba(OH)_2 solution. In addition, the pattern of precipitation of the non-biologically formed formazan was very similar to that of the biologically formed formazan. Accordingly, the substances which reduced TTC non-biologically were considered to be closely associated with the substrates for the microorganisms. (3) The plant debris stained with the red formazan could be mounted on a slide glass and observed at high magnification for the detailed study on the process of decomposition. Addition of lactophenol cotton blue to the plant debris could visualize the microorganisms responsible for the decomposition of the plant debris. (4) NTB (nitro tetrazolium blue) was the most suitable for staining individual microorganisms growing on cellulosic materials or plant debris. The double staining with NTB and rosebengal made the discrimination between dead or resting cells and viable ones possible when this technique was applied to the decomposed filter paper.
- 社団法人日本土壌肥料学会の論文
著者
-
Saito Masanori
Department of Internal Medicine (III), Kanazawa University School of Medicine
-
WADA Hidenori
Department of Agricultual Chemistry, The University of Tokyo
-
Wada Hidenori
Department Of Agricultual Chemistry The University Of Tokyo
-
Takai Yasuo
Department Of Agricultural Chemistry University Of Tokyo
-
Saito Masanori
Department Of Internal Medicine (iii) Kanazawa University School Of Medicine
-
Wada Hidenori
Department Of Agricultural Chemistry University Of Tokyo
-
Saito Masanori
Department Of Grassland Ecology National Institute Of Livestock And Grassland Science
-
Saito Masanori
Department Of Agricultural Chemistry The University Of Tokyo
関連論文
- Effect of Combined Therapy of Danaparoid Sodium and Tranexamic Acid on Chronic Disseminated Intravascular Coagulation Associated With Abdominal Aortic Aneurysm
- Dehydrochlorination of γ-Hexachlorocyclohexane (γ-BHC) by γ-BHC-Assimilating Pseudomonas paucimobilis(Microbiology & Fermentation Industry)
- Arbuscular Mycorrhizal Fungal Communities Associated with Some Pioneer Plants in the Lahar Area of Mt. Pinatubo, Philippines(Soil Biology)
- Semi-Quantitative Analysis of Arbuscular Mycorrhizal Colonization of Onion Roots Inoculated with Single or Mixed Species Based upon PCR-RFLP
- Effect of Concentration of Phosphate on Spore Germination and Hyphal Growth of Arbuscular Mycorrhizal Fungus, Gigaspora margarita Becker & Hall
- Effect of Phosphate Application to Arbuscular Mycorrhizal Onion on the Development and Succinate Dehydrogenase Activity of Internal Hyphae
- One Missense Mutation in the Factor X Gene Causing Factor X Deficiency - Factor X Kanazawa
- Short-term effects of fluvastatin, a hydroxylmethylglutaryl-coenzyme A reductase inhibitor, on coagulation and the fibrinolytic system in patients with hypercholesterolemia
- Cognitive resource model for the information-processing of task-irrelevant visual stimuli
- Effects of losartan, an angiotensin II receptor antagonist, on the fibrinolytic system in hypertensive patients
- B-12 Rapid Growth of Diamond-like Carbon Films by Nanopulse Magnetron Chemical Vapor Deposition(Session: Coatings/Thin Films)
- EFFECTIVENESS OF TETRAZOLIUM SALTS IN MICROBIAL ECOLOGICAL STUDIES IN SUBMERGED SOIL
- Immunohistochemical Evaluation of Giant Cell Tumor of Bone
- Synthesis of Diamond-Like Carbon Films by Nanopulse Plasma Chemical Vapor Deposition in Open Air
- EFFECT OF MOLECULAR HYDROGEN ON THE REDUCTION PROCESS OF SUBMERGED SOIL
- Factors affecting the occurrence of high-frequency positive occipital sharp transients of sleep
- Factors affecting the occurrence of high-frequency positive occipital sharp transients of sleep
- Rapid Growth of Diamond-like Carbon Films by Nanopulse Magnetron Chemical Vapor Deposition
- Origins of Nasal and Oral Staphylococci
- Synthesis of Diamond-Like Carbon Films by Nanopulse Plasma Chemical Vapor Deposition in Open Air
- New Primer Design for Identification of Oral rothia, Including R. aeria, Using Multiplex PCR