昆虫の表皮形成およびキチン合成に関する生理・生化学的研究
スポンサーリンク
概要
- 論文の詳細を見る
The tanned pupal cuticle was formed in vitro when the wandering stage larval epidermis of Manduca sexta was cultured in the presence of 5 μg/ml of β-ecdysone for 4 days. Since metamorphosis occurs in response to two releases of ecdysone, epidermis was explanted from feeding final-instar larvae (day 3, weighing 6.5-7.0g) before the first release of ecdysone and was cultured in Grace's medium. During the first 24 hr of exposure to 1μg/ml of β-ecdysone in vitro, the epidermis changed its cellular commitment to that for pupal cuticle formation. Juvenile hormone inhibited the change of the cellular commitment and the tissue subsequently formed larval cuticle in the presence of JH. The epidermis of the last instar larvae was found to convert JH I into JH-acid. This esterase activity increased as a function of larval age and was considered to be involved in elimination of intracellular JH from the target tissues in preparation for metamorphosis. The nuclei were isolated and assayed for specific JH binding using the competitive nuclear exchange assay. Specific binding was found only for epidermal nuclei from larvae before the change in commitment of the cells. Diflubenzuron was found to inhibit both endocuticle deposition and β-ecdysone-initiated pupal cuticle synthesis by using the in vitro culture technique. Both effects were due to its inhibition of chitin synthesis. When larvae were injected with ^<14>C-glucosamine after treatment of diflubenzuron (DFB), ^<14>C-UDP-N-acetylglucosamine (UDP-AGA) was found to accumulate in vivo. However, chitin synthetase was not inhibited by DFB. Peritrophic membrane in the midgut of Mamestra brassicae final instar larvae was used as a target to study the mode of inhibition of chitin synthesis by DFB. Chitin synthesis was not inhibited only when DFB and UDP-AGA were applied inside the midguts. When ^<14>C-AGA was applied outside the midguts, the amount of the radio-labeled compounds transported across the microvilli membrane was about 1/4 as much in the DFB-treated tissues as in the control tissues. The results lead the conclusion that DFB seems to be an inhibitor of UDP-AGA transport across the microvilli membranes.
- 日本農薬学会の論文
著者
関連論文
- ヨトウガ培養表皮の幼虫-蛹への転換に及ぼすエクダイソンと幼若ホルモンの作用
- 幼若ホルモン様物質 NC-170 のヨコバイ類の変態, 産卵, 胚子発生に対する影響
- 第 4 回国際農薬化学会議に出席して
- ヨトウガ終齢幼虫における 1-(3, 5-dichloro-2, 4-difluorophenyl)-3-(2, 6-difluorobenzoyl)-urea (CME-134) の表皮透過, 吸収, 移行
- 幼若ホルモン様物質, NC-170 のヒメトビウンカの変態および休眠に対する影響
- 将来の農業形態と農薬科学(21世紀農薬科学への提言, 30周年記念特別記事)
- GM 植物について考える
- カエル界の異変
- 福永一夫先生を悼む
- 昆虫の表皮形成異常に基づく昆虫成長調節剤
- 農薬製剤ガイド, 日本農薬学会, 農薬製剤・施用法研究会編, (1997), 日本植物防疫協会, 東京, 245pp., 3,600円
- プレコセン 2 のセジロウンカに対する三種の異なった作用
- 第 1 回国際農薬科学シンポジウム農薬・環境 : 分子生物学的アプローチ
- ウンカ, ヨコバイ類の細胞内共生微生物
- 昆虫成長制御剤に関する研究の動向
- 農薬と環境と安全性シンポジウム(第 1 回)
- 殺虫剤を中心とした研究の動向(第 6 回国際農薬化学会議(1986, オタワ)の紹介)
- 昆虫の表皮形成およびキチン合成に関する生理・生化学的研究
- 総務・登録
- 〔第5回国際農薬化学会議対策委員会〕各部門記録 (第5回国際農薬化学会議(5 ICPC)特集)
- ジミリンの作用点と作用機構(農薬の作用点と作用機構 (3))
- ワーゲニンゲン大学(オランダ)と ICI (イギリス)を訪問して(見学記)
- ベンゾイル尿素と昆虫表皮