カイコを用いた強制経口投与法によるBT剤の力価検定

概要

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The purpose of this paper is to point out that the potency of Bacillus thuringiensis insecticide can be accurately evaluated by using the force-feeding method and that the toxicity depends on the ambient temperature. The insecticide, Bacilex[○!R], prepared to achieve wide insecticidal spectrum and used in this experiments contained two varieties of Bacillus thuringiensis (var. aizawai and var. kurstaki). The force-feeding method which can be applied accurately with a micro applicator to 5th instar larvae of B.mori at the adequate dosage, enabled to evaluate quantitatively the potency of the insecticide and also, was more reliable and accurate than the poison diet method (method officially applied in Japan). Furthermore, mortality percentage increased gradually with the ambient temperature owing to the destruction of the mid-gut epithelium of B.mori caused by high temperature and/or long period of exposure. On the contrary, diet type or body weight of larvae did not affect the potency of the insecticide.
日本応用動物昆虫学会の論文
1979-08-25