高速液体クロマトグラフィーによる血清および肝組織中α-トコフェロール(ビタミンE)定量法の検討

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We established a HPLC method for determination of α-Tocopherol (Vitamin E) in serum and liver tissues. α-Tocopherol was extracted from serum and liver homogenate with a mixture of n-hexane and ethanol (4 : 1 v/v). The extracts were dried under gas flow of nitrogen at 40℃, and the residus were reconstituted with 200㎕ of 96% methanol. Twenty㎕ of the reconstituted specimen was injected into HPLC system equiped with reverse phase ODS column 150×4.6mm i.d., 20㎕ loop injector and fluorospectrophotometric detector (Ex.286nm, Em. 325nm) , and eluted with 96 % methanol. Within run precision, mean±SD and CV (obtained with this method) were 15.1± 0.36㎍/㎖, 2.4% for serum (n=15) and 23.9±1.20㎍/㎖, 5.0 % for liver tissues (n=10). The values of α-Tocopherol (mean±SD) in serum from healthy adults (n =20) and in liver tissues from mongrel dogs (n=-20) were 14.0±4.42㎍/㎖ and 20.7±7.41㎍/g , respectively. The method has an enough reproducibility and can be applied for determination of α-Tocopherol in biological materials in the field of laboratory medicine.