薄層クロマトグラフィーによる地衣成分の検出感度

概要

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1. The sensitivity of thin-layer chromatography is measured for the several lichen substances. 2. The minimum quantity of each lichen substance for chromatographic detection is as follows : 0.0025μg (lecanoric acid) ; 0.005μg (lobaric acid) ; 0.01μg (zeorin) ; 0.16μg (usnic acid) ; 0.3μg (vulpinic acid). 3. By usual microchemical extraction, 4.6% of dry weight of the thallus (Parmelia tinctorum Nyl.) can be obtained as the crude extract of lichen substances. The results for the other three lichens are as follows : Anzia colpodes (0.42%), Cladia aggregata (0.9%), Usnea diffracta (1.25%). In contrast, by using usual chemical extractors in the chemical laboratory, the crude extract (1.8g) of lichen substances was obtained from the extraction of the thalli (21g) of Usnea diffracta by acetone (8.6%). 4. The amount of the lichen substances can be estimated in some extent by the spot area on the chromatogram. 5. In the microchemical method, usually 2.5-5μg lichen substances are spotted on chromatograms ; therefore, if only one lichen substance is present in the acetone extract, the lichen substance can be detected without exception. However, if two or more lichen substances are present in the acetone extract, small amount of the lichen substance (i.e., less than 0.3μg in vulpinic acid, and less than 0.0025μg in lecanoric acid) may not be detected by the chromatography.
高知学園短期大学の論文
1977-04-30