<原著>マウス口蓋形成過程の組織化学的研究

概要

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The fusing palate of day 13-15 fetuses of ddYmice was examined by using the TUNEL (TdT -mediated dUTP-digoxigenin nick end labeling) method, immunofluorescent staining for cadherin (a cell adhesion molecule), and electron microscopy. Only few TUNEL-positive (apoptotic) cells were observed in the medial edge epithelium before the palatal shelves came into contact. After the epithelial seam was formed along the opposing palatal shelves and nasal septum, many TUNEL-positive cells appeared in the midline sheam, and in the nasal and oral epithelial triangles. There was E-cadherin in the epithelial cells of both the basal and superfi- cial cell layers, and P-cadherin in only the basal cell layers, before the opposing shelves came into contact. After the shelves adhered to each other, E-cadherin was present in all seam cells, and P-cadherin in only the basal layer cells of the seam. Some mesenchymal cells were observed by electron microscopy to be in direct contact with the basal epithelial cells of the seam through the basement membrane. It is supposed that cadherin has an important role during fusion of the medial edge epithelium of the opposing palatal shelves and that the fused midline seam is disrupted and disappears by apoptotic cell death.
北海道医療大学の論文
1999-12-30