<原著>細胞内TNFは口腔癌細胞のTNFアポトーシスシグナルに対して抵抗因子として働いている

概要

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The tumor necrosis factor (TNF) is known for potent antitumor activity both in vitro and in vivo. However, certain kinds of tumor cells are reported to be resistant even to TNF. Based on previous findings that endogenous TNF (en TNF) acts as an intracellular resistance factor against exogenous TNF by scavenging oxygen free radicals with induced manganous superoxide dismutase (MnSOD). The present study examined the involvement of caspase and en TNF in apoptotic cell death of TNF resistant oral squamous cell carcinoma cells (OSCC). The results were as follows : T. T cells were more TNF-highly sensitive than other OSCC ; T. T cells displayed lower expression of MnSOD activity and en TNF protein ; a nonsecretory human TNF gene transfected into T. T cells was highly MnSOD active and inhibited TNF -induced apoptotic cell death ; Parent T. T cells mediated TNF-induced apoptotic cell death by the reactive oxygen species (ROS) downstream caspase-3. These findings suggest that en TNF exerts a protective effect against TNF-induce apoptotic cell death by inducing MnSOD to scavenge ROS downstream caspase-3.
北海道医療大学の論文
2002-06-30