<原著>イソプロテレノールのマウス唾液腺コレステロール代謝におよぼす作用

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The purpose of this study is to investigate the effect of isoproterenol on cholesterol metabolism (in vitro and in vivo) in mouse salivary glands in comparision with liver and kidney. 1. In vitro, cholesterol biosynthesis in liver and salivary glands (submandibular plus sublingual) did not change. 2. In this in vivo experiment male ICR-JCL mice were injected with isoproterenol (0.3mg/mouse/day). By continued isoproterenol injections for 36 days, the total cholesterol levels in submandibular glands and kidney decreased, while cholesterol biosynthesis from acetate-1-^<14>C in submandibular glands and kidney increased. The submandibular gland was enlarged, however, kidney weight was slightly decreased. The total cholesterol level and biosynthesis in liver did not change. 3. By continuous isoproterenol injections for 45 days, the weight and the total cholesterol level of submandibular glands increased while cholesterol biosynthesis did not change. And after continuous isoproterenol injections for 45 days the uptake of cholesterol -1α, 2α (n)-^3H in submandibular glands decreased as dpm/g tissue, but did not change as dpm/gland/mouse in the following submandibular gland enlargement. 4. 21 days subsequent to the withdrawal of isoproterenol after continuous isoproterenol injections for 24 days, the weights of kidney and salivary glands (submandibular plus sublingual) returned to normal level, however, the total cholesterol level in salivary glands remained at an increased stage and cholesterol biosynthesis in liver and kidney also remained at a decreased stage. In brief, cholesterol metabolism did not return to normal condition even after 21 days subsequent to the withdrawal of isoproterenol after the isoproterenol injection. 5. The uptake of cholesterol-4-^<14>C or -1α, 2α(n)-^3H did not change in submandibular glands after isoproterenol injection for 2 or 3 days, however after continuous isoproterenol injections for 7 or 12 days it decreased as dpm/g tissue, but did not change as dpm/gland/mouse. The uptake of cholesterol in the subcellular fraction of submandibular glands increased in the microsome fraction, but decreased in the mitochondria fraction after isoproterenol injection for 12 days. As seen in the results above, during the salivary gland enlargement by isoproterenol, cholesterol biosynthesis increased, but in the maximum of salivary gland enlargement, cholesterol biosynthesis was stopped and returned to normal values. Following withdrawal after isoproterenol injection salivary gland enlargement returned to normal, but normalization of cholesterol metabolism occurrs to be later. These studies indicate that the source of cholesterol during salivary gland enlargement depends mainly on cholesterol produced by cholesterol biosynthesis.
北海道医療大学の論文
1984-06-30

<原著>イソプロテレノールのマウス唾液腺コレステロール代謝におよぼす作用

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