<原著>抗壁細胞抗体の測定法に関する研究 : 凍結切片法とエタノール固定パラフィン切片法の比較

概要

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We examined whether parietal cell antibodies could be detected in sera of 21 patients with Graves' disease by indirect immunofluorescence method on ethanol-fixed paraffin sections, using rat stomach as a target tissue. Also, we examined the parietal cell antibodies by frozen section method and compared both methods on staining appearance, staining procedure and parietal cell antibody titers. In ethanol-fixed paraffin section method, there was no autofluorescence. Parietal cell cytoplasma was stained homogeneously and parietal cell antibodies could be easily detected. But staining appearance, staining procedure and long-term preservation of sections were better in ethanol-fixed paraffin section method than in frozen section method. Parietal cell antibody titers measured on ethanol-fixed paraffin sections were positively correlated with parietal cell antibody titers measured on frozen sections (r=0.88,P<0.001). These results suggest that ethanol-fixed paraffin section method is an available method as for the measurement of parietal cell antibody titers comparable to frozen section method.
近畿大学の論文
1983-09-25