微量糖自動分析計の試作と各種降圧物質の構造研究への応用(A 基礎的研究)(II 予防および治療)

概要

論文の詳細を見る
An automated carbohydrate microanalyzer was produced on trial by assembling a liquid chromatography apparatus equipped with an automated solvent-exchange device and a data processor, a jacketed column packed with a high-quality resin, a parallel, double-plunger reagent pump, a mixer, a reaction bath, a sensitive fluorimetric detector, and a recorder. The carbohydrates eluted from the column were postlabelled with Fluoresugar, a newly developed fluorogenic reagent for carbohydrates, and the fluorescence was detected at 331 (excitation)/383 (emission) nm. This analyzer made it possible to determine a number of mono-and oligo-sacchari-des more than 10^<-11> mole in ca. 100 min. Pretreatment of samples was unnecessary. The carbohydrate compositions of the hypotensors isolated from various plants and fungi were analyzed by this instrument to give valuable information on their structures.
近畿大学の論文
1980-09-25