<Original>Micropropagation using Leaf Blades in Genus Lycoris
スポンサーリンク
概要
- 論文の詳細を見る
To develop an efficient tissue culture method for in vitro bulb formation from leaf explants of Lycoris, regeneration conditions were studied. Higher regeneration rates were obtained using 1 mg/l 1-naphthaleneacetic acid (NAA) with 5 mg/l 6-benzylaminopurine (BAP) in modified MS medium of Huang and Liu (1989). Adventitious buds were induced from the proximal (young) end of explant without intervening callus. Regeneration capacity was greatly affected by ages and parts of leaf, and the basal parts of younger leaves gave higher regeneration rates. In culturing the basal parts of younger leaves on the above optimum medium, 5.0-56.7% regeneration rates with 1.0-11.4 bulblets/explant were obtained in 12 out of 13 tested genotypes. Regenerated bulblets developed into whole plants with bulb after transfer onto hormone-free MS medium.
- 大阪府立大学の論文
- 2002-03-31
著者
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MORIKAWA Toshinobu
Graduate School of Life and Environmental Sciences, Osaka Prefecture University
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Ogawa Takeshi
Graduate School Of Life And Environmental Sciences Osaka Prefecture University
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Morikawa Toshinobu
Laboratory Of Genetics And Plant Breeding College Of Agriculture
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Morikawa T
Graduate School Of Life And Environmental Sciences Osaka Prefecture University
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Ma Biao
Laboratory Of Genetics And Plant Breeding Graduate School Of Agriculture And Biological Sciences Osa
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Ma Biao
Graduate School Of Life And Environmental Sciences Osaka Prefecture University
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Tarumoto I
Graduate School Of Agriculture And Life Sciences Osaka Prefecture University
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Tarumoto Isao
Laboratory Of Genetics And Plant Breeding Graduate School Of Agriculture And Biological Sciences Osa
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OGAWA Takeshi
Laboratory of Plant Molecular Improvement and Global COE Program, Graduate School of Nutritional and
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Ogawa Takeshi
Laboratory Of Plant Molecular Improvement And Global Coe Program Graduate School Of Nutritional And
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OGAWA Takeshi
Department of Pure and Applied Sciences, University of Tokyo
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OGAWA Takeshi
Department of Pure and Applied Sciences University of Tokyo
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OGAWA Takeshi
Laboratory of Genetics and Plant Breeding, Graduate School of Agriculture and Biological Sciences, Osaka Prefecture University
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